Stability-Indicating HPLC Method for Simultaneous Estimation of Sofosbuvir and Ledipasvir
The optimization of chromatographic conditions for the analysis of Ledipasvir and Sofosbuvir was successfully achieved using a Hibar C18(250×4.6mm)5µm column using 1% O-Phosphoric acid (pH 6.4): Acetonitrile (30:70 v/v) at a flow rate of 0.8 ml/min, ensuring excellent peak shape and resolution. System suitability tests confirmed compliance with essential parameters, including resolution, tailing factor, and […]
