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Protective effects of vitamin E and vitamin C against cisplatin nephrotoxicity in female Wister rats

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Cisplatin (CP) is a potent anticancer agent used in cancer treatment. However, Cisplatin had ability to induce multiorgan toxicity including nephrotoxicity. This study aims to investigate the effect of vitamin E (Vit. E) and Vitamin C (vit. C) on nephrotoxicity induced by cisplatin in female rats.

Twenty Female white albino rats were divided to four groups, control, cisplatin, vit. E + cisplatin and vit. C + cisplatin. Cispatin (8 mg/kg as a single dose on the day on and second dose at 14 experimental day.vit. E was administered Intraperitoneal injection 3 days prior to cisplatin (8 mg/kg, i.p) injection two does same process for two weeks with two-week rest period. vitamin C (200 mg/kg), were administered Intraperitoneal injection 3 days prior to cisplatin (8 mg/kg, i.p) injection two does same process for two weeks with two-week rest period. The result of this study administration of cisplatin induced Necrosis of epithelial cells of renal tubules in cortex area led to form spaces filled with inflammatory cells forming a cluster in affected areas in the cisplatin treated group compared with vit. E + Cisplatin treated group which reduced nephrotoxicity by antioxidant activity of vitamin against cisplatin induced oxidative stress in these rats. However, vit. C + Cisplatin treated group showed mild nephroprotection. Necrosis of epithelial cells was observed in renal tubules with infiltration of inflammatory cells that formed small cluster near the glomerulus in cortex area. The study findings indicated that vitamin E exert protective effects against CP-induced nephrotoxicity, a finding which requires larger studies for confirmation. cisplatin treatment alone induced the upregulation of cytokeratin-8 (CK-8), a marker of tubular injury, co-treatment with Vit.E attenuated this effect, indicating protection of renal tubular integrity. Also,cisplatin treatment group showed positive expression of vimentin in cortical convoluted renal tubules and normal expression in glomerulus.

Estimation of Polyphenol and Antioxidant Content from Papaya (Carica papaya) and Mango (Mangifera indica) Seed, Peel and Leaves

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Papaya (Carica papaya) and mango (Mangifera indica) are two tropical fruits which are widely known for their rich nutritional properties and various health benefits. Papaya belongs to family Caricaceae which has its origin in South Mexico. Mango belongs to the family Anacardiaceae and were originated from India and southern China. As the pulp of these fruits is known to be consumed rapidly while their seeds, peels and leaves are often thrown away as waste. This study involves the estimation of total polyphenol and anti-oxidant activity of the by-products of papaya and mango fruits. The anti-oxidant activity by DPPH method of % inhibition of papaya seed=180 peel=125 leaves=64 is higher compared to mango by-products. In FRAP, ABTS, Radical Cation method of mango samples shown higher levels than papaya. Vitamin c content of mango seeds exhibits high as 15.6mg/100g compared to papaya seed, papaya peel and leaves has higher levels of vitamin c compared to mango peel and leaves. The total polyphenol activity (GAE/g) of mango seed=118.4, peel=55.31, leaves=19.04 exhibits higher value compared to papaya. Papaya seed and peel has more beta carotene content compared to mango whereas mango leaves shown more beta carotene content than papaya. Seeds of papaya and mango has 2.05% and 2.15% pectin, mango peel has more pectin content than papaya 12.45%>8.94% and mango peel has shown slightly more pectin content than papaya as 4.25>3.58. Papaya peel have medium number of abundances of tannin content, seeds and leaves have less in number whereas mango have equal amounts of abundance of tannin content. This study mainly features the importance of the bioactive compounds found in the by-products (seed, peel, leaves) of papaya and mango and their health benefits.

Improving the Quality of Functional Food Crops and Land Efficiency by Intercropping System on Application of Integrated Fertilizer

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The intercropping system and integrated fertilizers were applied in the research to improve land efficiency and the quality of sweet corn and vegetable soybean crops. The study was conducted in the Giwangan Village of Umbulharjo Yogyakarta, Indonesia, and was set up using a three-replication factorial Randomized Complete Block Design. Three organic fertilizer sources (cow manure, chicken manure, and composted waste) made up the first factor, and the second factor was NPK fertilizer doses (200, 300, and 400 kg ha-1). Two control treatments were also made, which resulted in 33 experimental plots. The observed data were subjected to analysis of variance and Duncan’s Multiple Range Test at a significance of 5%. The study’s findings demonstrated that the combination of 300–400 kg ha–1 NPK fertilizer and chicken manure increased variables such as the content of vitamin C and pro-vitamin A, number of seed rows, number of 2-3 seed pods, and sweetness level of soybean and sweet corn, as well as the land equivalent ratio, land equivalent coefficient, competition index, system productivity index, and relative yield mixture.