GC-MS, FTIR, and HPLC analysis of Trichoderma sps, Rhizopus sps, and Aspergillus sps of Saraca asoca for anticancer molecules
Endophytic fungi combined with medicinal plants are reservoirs of therapeutic compounds. These endophytes are considered one of the major sources of bioactive molecules used in different aspects of health care. The present study attempted to assess the possibility of using the endophytic extract. The study showed a complete endophytic extract by using the GC–MS profile, indicating the presence of different volatile molecules in the endophytic extract. The HPLC fingerprint of S. asoca endophytic extract represents the characteristic markers of this herb. The endophytic extract’s HPLC showed caffeine, a Ret Time [min]- 3.052, and a Width [min]- 0.1322. The FTIR analysis of the functional groups present in the Trichoderma sps are =C-H (Alkynes, Amides), O-H (Carboxylic acids), C=C(Alkynes), C=C (Aromatic compounds), C-F (Alkyl and Aryl Halides). These functional groups are used in the production of antibiotics and antifungal drugs. The GC-MS study of the endophytic extract showed the presence of 59 compounds, which possessed anticancer properties. The anticancer activity was studied further.