Articles

Amino Acid Content in Black Soldier Fly (Hermetia illucens) Oil in the Larval, Prepupal and Pupal Phases as a Feed Additive in Broiler

/in /by

The global livestock sector is facing mounting pressure to adopt sustainable and efficient feed alternatives that can meet the growing demand for animal protein. Among the emerging options, Hermetia illucens (Black Soldier Fly, BSF) oil presents a promising candidate due to its bioactive compound richness and capacity to upcycle organic waste into nutrient-dense biomass. This study investigates the essential and non-essential amino acid composition of BSF oil extracted from larvae (8 days), prepupae (14 days), and pupae (28 days) stages and evaluates its potential as a feed additive in broiler production. High-Performance Liquid Chromatography (HPLC) revealed significant variations across developmental phases, with larval oil exhibiting the highest lysine (4,467.56 mg/100g) and glutamic acid (7,564.45 mg/100g), while pupal oil was enriched in leucine (5,670.76 mg/100g) and tryptophan (4,356.79 mg/100g). The dynamic amino acid profile supports the inclusion of BSF oil as a stage-specific supplement in poultry feed, offering both nutritional and economic advantages. Findings from this study contribute to the growing body of knowledge on insect-based feed ingredients and provide critical insight for formulating phase-targeted broiler diets with optimized functional performance. However, essential amino acids such as leucine, lysine, and tryptophan remained stable or even increased. Among all stages, larval oil exhibited the richest amino acid composition, suggesting its suitability for use in high-performance starter feeds for broilers.

The Addition of Lauric Acid from DPKFA (Distilled Palm Kernel Fatty Acid) as A Feed Additive on The Physiological Responses of Broiler

/in /by

The aim of the research is to examine the effect of adding additive products to feed on the physiological responses of broilers during 1 (one) rearing period including rectal temperature, shank temperature, comb temperature, respiratory frequency and heart rate frequency. The research was conducted from September 21 to October 26, 2024 in Mr. Soleh’s Farm. Kidal Village, Tumpang District, Malang Regency, East Java. The material used in this study was non-sexing DOC (Day Old Chick) strain Lohmann from PT Japfa Comfeed Tbk. A total of 300 broilers with a body weight diversity coefficient of less than 10% were reared for 35 days. This study used 6 treatment groups, each consisting of 5 replicates including T0 (Basal feed as control treatment), T1 (Basal feed + zinc bacitracin 0.1% as positive control treatment), T2 (Basal feed + 0.05% feed additive product), T3 (Basal feed + 0.10% feed additive product), T4 (Basal feed + 0.15% feed additive product) and T5 (Basal feed + 0.20% feed additive product). The data obtained were analyzed using analysis of variance (ANOVA) and continued with Duncan’s Multiple Range Test (DMRT) if there was a significant effect. The results showed that the addition of DPKFA-based feed additive products with high lauric acid content in the feed did not significantly affect the physiological response of broilers (P>0.05). The provision of additives with the highest concentration helps to optimize the physiological response of broilers so that this product can be recommended as a substitute for the role of AGP to support the performance of broiler production and health.

Improving Reproductive Performance of Laying Hens with a Combination of Selenium: Effects on FSH and LH Hormones

/in /by

This research aims to evaluate the provision of inorganic and organic selenium in feed on the reproductive status of layers. This research took the main object as layers aged 60 weeks with the ISA Brown strain. The research used a Completely Randomized Design (CRD) method with Duncan’s advanced test. The results of this study explain that giving additional feed in the form of selenium (Se) with several doses of basal feed plus inorganic selenium 1000 g/ton of feed and organic selenium 225 g/ton of feed showed a significant increase in the FSH hormone, while giving basal feed plus inorganic selenium at a dose of 2,500 g/ton of feed and organic selenium at a dose of 375 g/ton of feed gave a significant increase in the LH hormone. The best treatment results are because the additional feed contained in organic selenium can help layers improve reproduction to produce good quality eggs.

Effect of the Addition Red Beetroot Powder (Beta vulgaris L. Var. Rubra L) as a Feed Additive in Feed on Production Performance, Egg Yolk Cholesterol and Blood Profile of Laying Hens

/in /by

The aim of this research was to evaluate the effect of addition red beetroot powder (Beta vulgaris L. Var. Rubra L) as a feed additive in feed on production performance, egg yolk cholesterol and blood profile of laying hens. The first stage of research included testing the inhibitory power of Escherichia coli, Salmonella sp, and Lactic Acid Bacteria (LAB). The materials used in the bacterial inhibition test were red beetroot pulp powder, red beetroot peel powder and red beetroot pulp and peel powder. The method used was in vitro research and the data was analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. The second stage of research is the field application of the results of the first stage research in vivo. The material used was 120 laying hens of the isa brown strain aged 30 weeks. The method used was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 6 replications, each using 5 chickens. Data were analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. Variables observed in the second stage of research included production performance, egg yolk cholesterol and blood profile of laying hens. The results of the first stage of research showed that the use of red beetroot plant powder had a very significant effect (T<0.01) on the diameter of the inhibition zone for Escherichia coli, Salmonella sp and Lactic Acid Bacteria (LAB). Red Beetroot Pulp and Peel Powder (RBPPP) has the best antibacterial power so it was applied in the second stage of research. The results of the second stage of research showed that the addition of RBPPP had a significant effect (T<0.05) on feed consumption, had a very real effect (T<0.01) on egg weight, HDP, egg mass, feed conversion, IOFC, hemoglobin., erythrocytes and hematocrit. However, there was no significant effect (T>0.05) on egg yolk cholesterol and leukocytes. The addition of RBPPP at the 1% level has the potential to reduce feed consumption and feed convertion ratio, increase egg weight, HDP and egg mass, even though the IOFC value is lower than the control treatment or without RBPPP. The addition of RBPPP at a level of 1.5 % has the potential to reduce egg yolk cholesterol levels compared to other treatments. The addition of RBPPP up to a level of 1 % is able to increase and maintain hemoglobin and hematocrit levels in normal conditions. Apart from that, the addition of RBPPP at levels of 0.5 % and 1.5 % is able to increase and maintain erythrocyte and leukocyte levels in normal conditions.