Articles

Analysis of Chloramphenicol in Shrimp Using Standard Addition Method Based on Diazotization

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This research aimed to determine the concentration of chloramphenicol in shrimp using the standard addition method based on the diazotization reaction using Zn powder as a reducing agent of chloramphenicol, followed by the use of N-(1-naphthyl) ethylenediamine dihydrochloride as a coupling agent and measured at 565 nm. Based on the test, the shrimp sample was found to contain 1964.91 mg/kg of chloramphenicol and it exceeded the requirements set by the European Commission which was 0,15 mg/kg. The limit of detection (LOD) value is 0.19 mg/mL and the limit of quantitation (LOQ) value is 0.64 mg/mL. The correlation coefficient (R2) was 0.9991 for the concentration range of 0-50 ppm. The analysis of the results showed that the %recovery in shrimp analysis using this method was 87.41%-107.73% with an average of 109.38%.

The Effect of Variations Concentration of Chloramphenicol on the Imprinting Factor of Molecularly Imprinted Polymer

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This research aimed is to determine the effect of chloramphenicol concentration on the imprinting factor (IF) value of the Molecular Imprinted Polymer (MIP). Imprinting Factor is a standard of the interaction power between printed polymer and template molecule. The IF value was calculated based on the adsorption capacity value between MIP and blank polymer (BP). MIP was synthesized from non-imprinted polymer (NIP) using the precipitation method with chloramphenicol as a template, methacrylic acid (MAA) as a monomer, and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The results showed that the optimum concentration was at 10 ppm with the IF value of 5,005. The isothermal adsorption result of Chloramphenicol using MIP can best be described by the Langmuir model. The limit of detection (LOD) value was 0.098 and the limit of quantification (LOQ) value was 0.327.