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The Influence of Lipase Enzyme Level and Ca(OH)2 Usage in the Production of Protected Lemuru Fish Oil Supplement on the Texture, Nutrient Content, and Fatty Acid Value of the Product

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The scarcity of  feed energy source to ruminant can be anticipated by adding oil to the feed. However, the effect of adding oil to the feed more than 7% will reduce the activity of fiber-degrading microbes because fat is toxic. In addition, fat in the feed will easily undergo biohydrogenation by rumen microbes so that it will reduce its function. Making fat protection feed using lemuru fish oil with a combination of lipase enzymes and Ca(OH)2 can be a solution because it aims to protect fat from biohydrogenation in the rumen. The experiment used a completely randomized design method with a 3×2 factorial pattern with factors 1) lipase enzyme (0%, 0.5%, and 1%) and factor 2) Ca(OH)2 (20% and 25%). The product results obtained showed that the product quality was in good condition with the product hardness criteria at number 4 (hard). Analysis of variance showed no significant effect on interaction,enzyme factor and Ca(OH)2 factor (P>0.05) on DM, OM, CF and acid number where the combination of L1K20 treatment (1% enzyme and 20% Ca(OH)2) produced the highest DM content of 90.25±1.12%; OM 81.98±1.35%; CF 14.62±0.53%; and the lowest acid number of 0.54±.0.06 mg KOH/g sample. The conclusion that can be given is that the use of a combination of 1% lipase enzyme and 20% Ca(OH)2 in fat protection products produces good quality products in terms of product hardness, high nutrient content and low acid number according to the standard fatty acid number below 1 mg KOH/g sample.