Effect of Black Garlic Extract on Aspirin-Induced Gastric Mucosal Injury in Wistar Rats (Rattus norvegicus)
Background: Nonsteroidal anti-inflammatory drugs (NSAIDs), including aspirin, are widely used but are associated with gastric mucosal injury through inhibition of prostaglandin synthesis and oxidative stress mechanisms. Black garlic, a processed form of Allium sativum, has been reported to possess antioxidant and anti-inflammatory properties that may provide gastroprotective effects. Objective: To evaluate the effect of black garlic extract on aspirin-induced gastric mucosal injury in Wistar rats (Rattus norvegicus). Methods: This experimental laboratory study used a posttest-only control group design. Male Wistar rats were allocated into a negative control group, a positive control group receiving aspirin, and three treatment groups administered black garlic extract at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight. Aspirin was administered orally at 104 mg/kg body weight for seven days to induce gastric mucosal injury, followed by seven days of black garlic extract administration in the treatment groups. Gastric tissues were collected for histopathological examination, and epithelial injury was assessed using the Barthel Manja scoring system. Data were analyzed using non-parametric statistical tests.
Results: Histopathological evaluation demonstrated gastric mucosal epithelial injury in the positive control group, predominantly in the form of desquamation and erosion. Administration of black garlic extract was associated with reduced severity of mucosal injury, with progressive improvement observed at higher doses. The group receiving 200 mg/kg body weight showed no detectable gastric mucosal lesions. A significant difference in gastric mucosal injury was observed among groups (p<0.001).
Conclusion: Black garlic extract exhibited a protective effect against aspirin-induced gastric mucosal injury in Wistar rats, with the highest dose demonstrating the most favorable histopathological outcome.