Articles

Bioremediation of Saline Soils with the Consortium of Halophilic Bacteria

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88 strains of bacteria were isolated and purified from the naturally saline soils of Kumisi, Sagarejo and Alazani valley (Eastern Georgia). Based on the initial identification all strains belong to the genus Bacillus. Two strains – Bacillus spp. 8(3)1) and Bacillus spp. 7(5)4, resistant to chloride, sulfate and mixed (chloride-sulfate) salinity, as well as possessing high cellulase and amylase activities were selected as a result of successive screenings among the isolated bacteria. After the antagonism between potential members of the consortium has been excluded, the consortium of halophilic bacteria was created. Compost was made and applied to moderately saline soils using the halophilic consortium. The bioremediation of saline soils of Alazani was especially successful: the halophilic consortium has reduced the salt content in the soil by 41% during the 21 days of composting.

Biodegradation of Polycyclic Aromatic Hydrocarbon Compound by Bacterial Cultures

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In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene by four bacterial cultures Bacillus subtilis PD6, Bacillussp. PD9, Enterobactersp. PD11 and Bacillussp. PD14 has been targeted. Biodegradation of Naphthalene by these four selected bacterial cultures was analysed by HPLC (High Performance Liquid Chromatography) technique. HPLC analysis revealed biodegradation of naphthalene by all the four bacterial cultures within a span of six days. Highest biodegradation 78.1% has been shown by Bacillus subtilis PD6 while other bacterial cultures Bacillus sp. PD9 has shown 77.90%, Enterobacter sp. PD11 showed 74.4% and Bacillus sp. PD14 exhibited 73.5% biodegradation of naphthalene.