Articles

Effect of Stirring Circulation on the Effectiveness of Microbial Consortium in Sludge Oil Bioremediation Process

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Sludge oil is a by-product of crude oil processing that poses significant environmental and efficiency challenges in the oil and gas industry. This study aims to evaluate the effectiveness of using microbial consortia to address sludge oil, with a focus on the impact of agitation on oil recovery enhancement. An experimental and laboratory analysis approach was employed to implement bioremediation for cleaning sludge oil from crude oil tanks. Factors such as agitation conditions, types of microorganisms, environmental conditions, and handling time were evaluated to understand their effects on oil recovery efficiency. The experiment compared two conditions: microbial consortia in agitated and non-agitated systems. The results showed that the use of microbial consortia in an agitated system was significantly more effective in reducing sludge oil and enhancing oil recovery compared to the non-agitated system. Agitation was found to improve microbial circulation and nutrient distribution, contributing to an increase in sludge oil degradation by up to 17.1%. However, the success of this method was also influenced by the composition of the sludge oil and environmental conditions. This research contributes to the development of more effective solutions for managing sludge oil and improving oil recovery in the oil and gas industry. The results of this experiment can serve as a foundation for further research on optimizing bioremediation for different operational conditions and supporting environmental sustainability.

Bioremediation of Saline Soils with the Consortium of Halophilic Bacteria

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88 strains of bacteria were isolated and purified from the naturally saline soils of Kumisi, Sagarejo and Alazani valley (Eastern Georgia). Based on the initial identification all strains belong to the genus Bacillus. Two strains – Bacillus spp. 8(3)1) and Bacillus spp. 7(5)4, resistant to chloride, sulfate and mixed (chloride-sulfate) salinity, as well as possessing high cellulase and amylase activities were selected as a result of successive screenings among the isolated bacteria. After the antagonism between potential members of the consortium has been excluded, the consortium of halophilic bacteria was created. Compost was made and applied to moderately saline soils using the halophilic consortium. The bioremediation of saline soils of Alazani was especially successful: the halophilic consortium has reduced the salt content in the soil by 41% during the 21 days of composting.

Biodegradation of Polycyclic Aromatic Hydrocarbon Compound by Bacterial Cultures

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In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene by four bacterial cultures Bacillus subtilis PD6, Bacillussp. PD9, Enterobactersp. PD11 and Bacillussp. PD14 has been targeted. Biodegradation of Naphthalene by these four selected bacterial cultures was analysed by HPLC (High Performance Liquid Chromatography) technique. HPLC analysis revealed biodegradation of naphthalene by all the four bacterial cultures within a span of six days. Highest biodegradation 78.1% has been shown by Bacillus subtilis PD6 while other bacterial cultures Bacillus sp. PD9 has shown 77.90%, Enterobacter sp. PD11 showed 74.4% and Bacillus sp. PD14 exhibited 73.5% biodegradation of naphthalene.