Articles

Finding the Therapeutic Role of miRNAs in Hepatocellular Carcinoma (HCC)

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Hepatocellular carcinoma (HCC) has become the second most common cause of cancer-related death worldwide, with approximately 782,500 new cases and 745,500 deaths occurring in the global during 2012. The main objective of the study is to find the therapeutic role of miRNAs in Hepatocellular Carcinoma (HCC). This study was conducted using a systematic search on Google scholar, Pubmed and Web of science published until 20th June 2020. The cited references of retrieved articles and previous reviews were also manually checked to identify any additional eligible studies. MiRNAs perform their tumor suppressor functions through downregulating oncogene expression. Increasing evidence suggests that miRNAs are essential for the regulation of liver development, regeneration, and metabolic functions. It is concluded that deregulation of miRNAs significantly contributes to the development of HCC. miRNAs mainly functions to down regulate the expression of targeted genes. However, they may have other yet unknown functions including the activation of gene transcription.

 

Protective Effect of Selenium against Methotrexate Induced Hepatotoxicity

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Introduction: The liver is the largest internal organ by percent weight in the human body and has crucial functions, including cholesterol production, intermediary metabolism, hormone synthesis, bile and urea production and drug detoxification.
Objectives: The main objective of the study is to analyse the Protective effect of selenium against methotrexate induced hepatotoxicity in mice.
Material and methods: This descriptive study was conducted in Jinnah Hospital Lahore during 2020. Selenium and all chemical reagents of analytical grades were purchased from Sigma Chemical Co. (St. Louis, Mo, USA). The dose of MTX diluted in water (50% 1ml/kg of body weight of Mice were applied twice a week for six weeks to induced hepatocellular injury determined by liver function tests. The mice were maintained under pathogen-free conditions with air conditioning, a 12-hr light/12-hr dark cycle, and %55 humidity.
Results: Results shows that a significant increase in serum enzymes (ALT, AST, ALP) and significant decrease in total protein was observed in all groups receiving MTX for the induction of hepatic injury. Mice receiving combination therapy of selenium along with MTX shows a time course recovery towards normalcy. The highest value of ALT (94.83 IU/L), AST (73.21 IU/L) and ALP (157 IU/L) were recorded in Mice receiving MTX for hepatic injury. The lowest values (63.67, 51.49 and 139.95 IU/L) was recorded in group treated with MTX+ Se (200mg/kg b.w) but both the groups differed non significantly and shows the same trend but a decreasing trend in serum enzymes (ALT 32.85%), (AST 29.67%) and (ALP 11.40%).
Conclusion: It is concluded that selenium supplementation in MTX treated rats elicited a reduction in the toxic effects of the pesticide by improving the studied parameters, which was confirmed by the biochemical analysis of serum.