Articles

The Potential of Daruju Leaf Extract (Acanthus Ilicifolius) as a Bioreductor in the Synthesis of Silver Nanoparticles and Antibacterial Activity Test

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Silver nanoparticles had been synthesized through the bioreduction process using daruju (Acanthus ilicifolius) leaf extract as a bioreductant. The variation of the formula used is a mixture of daruju leaf extract with a 0.01 M AgNO3 solution with volume ratios of 2:1, 1:1, 1:2, and 1:3. The research results show that UV-Visible spectrophotometry confirmed that a volume ratio of 1:1 is the optimum formula with an absorption value of 2.346 at the maximum wavelength 440 nm. Synthesis of silver nanoparticles with daruju leaf extract has a strong inhibition zone about 10.42 mm. Proving that the synthesis of silver nanoparticles with extract daruju leaf can be used as an antibacterial agent against E.coli bacteria.

Royal Jelly (Apis mellifera) As an Antimicrobial Activity Inhibition

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Royal jelly (RJ) has lots of compounds such as protein, vitamins, and a variety of bioactive compounds (10-hydroxy-2-decanoic or 10-HDA acids). 10-HDA is a lipid fraction component in RJ content with antibacterial and immunomodulatory activity. RJ proteins that have antimicrobial, antifungal, anti-tumor, antidiabetic, and antihypertensive properties. The authors continue their research about the effect of RJ on the inhibitory strength of the given antibacterial activity. L. monocytogenes, S. aureus, and E. coli were among the microbes studied in this research. This study used an experimental method with a complete randomized design (CRD) of 5 treatments and 4 replications, if the treatment showed a noticeable effect (P<0.05) then Duncan’s multiple distance test was continued. According to the findings of this study, the royal jelly variations given had a significant inhibitory effect on antibacterial activity. The inhibitory ability of L. monocytogenes proved that the second treatment had a higher inhibitory power of 30.94±25.786b, in S. aureus, the second treatment gave a moderate inhibitory power of 9.013±1.841a, and in E. coli, the second treatment gave a weak inhibitory power of 3.24±3.748a. The conclusion of studies showed royal jelly with dilution variety could be antimicrobial agent and to be strongest inhibitor in L. monocytogenes and lowest in E. coli. The suggestion of studies needs management in the isolates of bacteria available and should be estimated before we do the research about antimicrobial activity inhibitors.