Abstract :
This study was conducted to examine the effect of various kinds of Moringa leaf extracts on cholesterol, estradiol, FSH, LH levels and litter size in rabbit does. The extracts used were hexane fraction extract (HeEF), ethanol fraction extract (EtEF) and ethanol rough extract (EtCE) from moringa leaf flour. Seven treatments were administered including extracts in pellets at a dose of 0, 0.13% HeEF, 0.26% HeEF, 0.37 EtEF, 0.74% EtEF, 0.54% EtCE and 1.08% EtCE. Each treatment was reduplicated on 4 rabbits. Cholesterol levels, estradiol, LH and FSH blood serum, mating readiness and litter size were measured, which results showed that the treatment significantly (P <5%) lowered the cholesterol level and increased the estradiol level in blood serum. On the other hand, the treatment had no significant effect on FSH, LH and litter size levels except at LH levels 2 hours after mating.
Keywords :
Cholesterol, Estradiol, Estrogen, FSH, LH, Moringa Leaf Extract., RabbitReferences :
1. Ryokkynen, A., 2006. Effects of Phytoestrogens on Reproduction and Weight Regulation of Mammals. Dissertations in Biology. . University of Joensuu; 13-21.
2. Potocka, W.B., C. Mannelli, D. Boruszewska, I. Lowalczyk-Zieba, T. Wasniewski, and D. J. Skarzynski. 2013. Diverse Effects of Phytoestrogens on the Reproductive Performance: Cow as a Model. nternational Journal of Endocrinology. Volume 2013, Article ID 650984, http://dx.doi.org/10.1155/2013/650984
3. Pawiroharsono, S. 2001. Prospekdan ManfaatIsoflavonuntuk Kesehatan. Direktorat Teknologi Bioindustri, Badan PengkajiandanPenerapanTeknologi; 1-8.
https://www.scribd.com/doc/117479146/Prospek-Dan-Manfaat-Isoflavon-Untuk-Kesehatan. 12 Pebruari 2016
4. Mutiara, K.T, Harijono, T. Estiasih, E.S. Wahyuni. 2013. Effect LactagogueMoringa Leaves (MoringaoleiferaLam) Powder in Rats White Female Wistar, J. Basic. Appl. Sci. Res., 3(4)430-434.
5. Karthivashan, G., P. Arulselvan, A.R. Alimon, I.S. Ismail, and S.Fakurazi. 2015. Competing Role of Bioactive Constituents in Moringaoleifera Extract and Conventional Nutrition Feed on the Performance of Cobb 500 Broilers, BioMed Research International 2015; .Hindawi Publishing Corporation. Doi:10.1155/2015/970398
6. Setiasih, Wahjuningsih Sri, Winarsih Sri, Soetantob Hendrawan, 2019. The Effects Of Adding MoringaOleifera Leaves Extract On Rabbit Does’ Milk Production And Mammary Gland Histology. Rjoas, 8(92), August 2019. Doi 10.18551/Rjoas.2019-08.34: 296-304
7. Fahey, J.W. 2005. Moringaoleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1, Johns Hopkins School of Medicine, Department of Pharmacology and Molecular Sciences, Lewis B. and Dorothy Cullman Cancer Chemoprotection Center, 725 N. Wolfe Street, 406 WBSB, Baltimore, Maryland, USA 21205-2185.
8. Estrella, MCP., M.D., Jacinto Blas V., Mantaring III, M.D., G.Z. David, M.D., Michelle A. Taup, M.D. 2000. A Double-blind, Randomized controlled trial on the use of malunggay (Moringaoleifera) for augmentation of the volume of breastmilk among non-nursing mothers of paterm infants, Galacto.Com. Philippine Journal Pediatry49: 3-6.
9. Ola S.I., O.S. Williams, I.A. Obamojure, A.M. Okunlola, 2012. Sexual Receptivity And Conception Rate Of Rabbit Does Fed Selected Perennial Forages In Ile-Ife, Nigeria. Proceedings 10 Th World Rabbit Congress – September 3 – 6, 2012– Sharm El- Sheikh –Egypt, 291 – 295
10. Nath, D., N. Sethi, R.K. Singh, A.K. Jain, 1992. Commonly Used Indian Abortifacient Plants with Special Reference to Their Teratologic Effect in Rats. Journal of Ethnopharmacology 36 (2); 147-154.
11. Raj, A., A.Singh., A. Sharma, N. Singh, P. Kumar, V.Bhatia. 2011. Antifertility Activity of Medicinal Plants on Reproductive system of Female Rat. International Journal of Bio-Engineering Sciences & Technology-IJBEST.http://technicaljournals.org. 2 (3): 44-50. 4 Maret 2015.
12. Alemede, I.C., E.A. Onyeji, D.N. Tsado, E.L. Shiawoya. 2014. Reproductive Response of Rabbits Dose To Diets Containing Varying Levels of Horseradish (Moringa oleifera) Leaf Meal, Journal of Biology, Agriculture and Healthcare. ISSN 2224-3208 (paper) ISSN 2225-093X (online). 4 (19) :62-68, 22 Mei 2015.
13. Ayodele, A.E., J.R. Adeola, A.T. Mayowa. 2014. Reproductive Performance of Rabbit Does Fed Graded Levels of MoringaOleifera Leaf Meal Based Diet, International Journal of Science.(3): 49-53
14. Ara, N., Rashid, M. and Amran, M.S. 2008. Comparison of Moringa oleifera Leaves Extract with Atenolol on Serum triglyceride, Serum Cholesterol, Blood glucose, heart weight, body weight in Adrenaline Induced Rats. Saudi J. Biol. Sci., 15, 253-258.
15. Asiedu-Gyekye, I.J, S Frimpong-Manso, C. Awortwe, D.A. Antwi, and A.K. Nyarko. 2014. Micro- and Macroelemental Composition and Safety Evaluation of the NutraceuticalMoringaOleifera Leaves, Journal of Toxicology 2014. HindawiPublishing Corporation: 786979. doi:10.1155/2014/786979.
16. Nguyen, T.T, 1999. The Colesterol-lowering Action of Plant Stanol Esters. American Society for Nutritional Science.129: 2109 – 2112.
17. Yu-Yang, R., L. Chang, J. Hsu, B.B.C.Weng, M.C. Palada, M.L. Chadha and V. Levasseur, 2006. Nutritional and Functional Properties of Moringa Leaves− FromGermplasm, to Plant, to Food, to Health. Moringa and other highly nutritious plant resources: Strategies, Standards and Markets for a Better Impact on Nutrition in Africa. Accra, Ghana, November 16-18, 22.
18. Berne, R.M., M.N. Levi. 1990. Priciples of Physiology. USA C.V. Mosboy Co; 122-178
19. Lilibeth, A.C. and L.P. Glorina, 2010. Effects Moringaoleifera lam (Moringaceac) on The Reproduction of Male Mice (musmusculus), J. Med. Plant Res. 4(12): 115-121.
20. Lebas, F, P. Coudert, R. Rouvier, H. de Rochambeau, 1986. The Rabit. Husbandry, Health and production. Food and Agriculyural Organization of United Nations. Rome; 21-65
21. Saleh, A.M., 2013. Histological Study Of Ovary Through Last Periods (Oryctolagus Cuniculus) Of Pregnancy In Domestic Rabbit. Veterinary Medical Sciences Vol. (4) 11-19
22. Gómez, R.B., PCM. Becerril, H.G. Torres, and M.A Pro. 2004. Relationship among Feeding Level, Change of Cage and Fasting with Vulva Color and Sexual Receptivity in New Zealand White and Californian Nulliparous Does., Proceedings of the 8th World Rabbit Congress, no. 1978: 270–275.
23. Roland, W.S.U.,. R.J. Gouka, H. Gruppen, M. Driesse, L. van Buren, G. Smith and J. Vinken, 2014. 6-Methoxyflavanones as Bitter Taste Receptor Blockers for hTAS2R39. PLOS one. (9) : 1-10
24. Rosenthal, K. 2002. Blood Chemistry Ranges of Rabbits. Universiyy of Pennsylvania of Veterinary Medicine Faculty. www. Mey, 10, 2017; 1-3
25. Xinmei, X., X. Ning, H. Jianbin. 2015. Physiological Function of Phytosterol and Its Application. Animal Husbandry and Feed Science 7 (2): 67–69. doi:10.1007/s13398-014-0173