Abstract :

Type 2 diabetes mellitus is the most common type of diabetes, having high blood glucose, reduced insulin secretion and / or inadequate glucagon secretion. The study is to assess the activities of pancreatic enzymes and anthropometric indices. 81 participants comprised 38 diabetic and 43 control were randomly recruited. Informed, oral and written consent was obtained from the participants. Ethical approval was obtained.6mls of fasting blood samples were collected. Plasma glucose; amylase and lipase activities were analyzed using standard laboratory methods. Body mass index (BMI) of participant was determined from height and weight. The results showed significantly increased plasma glucose level in the diabetic participants than in control at p=0.000 and in male diabetics than in female diabetics at p=0.048 in each case. But the activities of lipase, amylase, the mean age and BMI level were the same in both diabetic and control groups at p>0.05 respectively. BMI level, lipase and amylase activities were the same in both genders (p>0.05). Stronger positive correlation exist between Weight Vs BMI (r=0.834; p=0.000), followed by Lipase Vs Amylase (r=0.767; p=0.000), least between Age Vs BMI (r=0.353; p=0.022) but weaker negative associations exist between Height Vs BMI (r=-0.490; p=0.001) and Weight Vs FBS (r=-0.325; p=0.036) in the diabetic subjects. The significant higher level of blood glucose; stronger positive correlation between Lipase and Amylase; Weight and BMI may likely revealed pancreatic exocrine function abnormality in diabetes mellitus type 2.

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Keywords :

Amylase, Diabetes, Glucose, Lipase, Mellitus, Type 2

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References :

1. Muanya C. 6m Nigerians live with diabetes as disease kills over 40,000. 2016, [cited 2021 July 28]. Available from: https://guardian.ng/news/1-6m-nigerians-live-with-diabetes-as-disease-kills-over-40000/.
2. World Health Organization. Diabetes country profile: Nigeria. 2016, [cited 2021 July 28]. Available from: http://www.who.int/diabetes/countryprofiles/nga_en.pdf?ua=1.
3. Ajikobi D. Does Nigeria have the most people with diabetes in sub-Saharan Africa?. 2018, [cited 2021 July 28]. Available from: https://africacheck.org/reports/nigeria-people-diabetes-sub-saharan-africa/.
4. Uloko AE, Ofoegbu EN, Chinenye S, Fasanmade OA, Fasanmade AA, Ogbera AO, et al. Profile of Nigerians with diabetes mellitus – Diabcare Nigeria study group (2008): Results of a multicenter study. Indian J Endocrinol Metab. 2012;16(4):558-64. doi: 4103/2230-8210.98011. [PubMed: 22837916]. [PubMed Central: PMC3401756].
5. Yang, J. J. (2019). Association of diabetes with all-cause and cause-specific mortality in Asia. Journal of American Medical Association Network Open; 2:e192696.
6. Policardo, L. (2015). Effect of diabetes on hospitalization for ischemic stroke and related in-hospital mortality: a study in Tuscany, Italy, over years 2004–2011. Diabetes Metabolism and Research Reviews; 31:280–86.
7. Bragg, F. (2017). Association between diabetes and cause-specific mortality in rural and urban areas of China. Journal of American Medical Association; 317:280–89.
8. Subedi, S., Shrestha, L., Gurung, K., Adhikari, B., Dhakal, G. (2020). Status of Serum Amylase in type 2 Diabetic Patients in Pokhara, Nepal. Journal of Hematology and Allied Sciences; 10(2):63-7.
9. Islam, S., Rahman, S., Haque, T., Sumon, A.H., Ahmed, A.Z.M., Ali, N. (2020). Prevalence of elevated liver enzymes and its association with type 2 diabetes: A cross-sectional study in Bangladeshi adults. Endocrinology, Diabetes and Metabolism; 3:e00116.
10. Bosco, D. (2010).Unique arrangement of alpha-and beta-cells in human islets of Langerhans. Diabetes; 59(5):1202-10.
11. Campbell, T. (2012). Pancreas organ weight in individuals with disease associated autoantibodies at risk for type 1 diabetes. Journal of American Medical Association; 308(22):2337-39.
12. Crook, M.A. (2012). Alkaline phosphate. In: Clinical Biochemistry and Metabolic Medicine Hodder and Stoughton Ltd, London, 8^th Edition, Pp. 276-8.
13. Williams, A.J. (2012). Pancreatic volume is reduced in adult patients with recently diagnosed type 1 diabetes. Journal of Clinical Endocrinology and Metabolism; 97(11):E2109-13.
14. Naing L, Winn T, Rusli B (2006). Practical issues in calculating sample size for prevalence studies. Archives of orofactual science. (1): 9-14.National AIDS Control Agency (NACA) (2011). Report on HIV prevalent rate of 3.1% in Nigeria.
15. Kurooka, S., Kitamura, T. (1978). Properties of serum lipase in patients with various pancreatic diseases. Analysis by a new serum lipase assay method (the BALB-DTNB method) in combination with gel-filtration and iso-electrofocusing techniques. Journal of Biochemistry; 84:1459-66.
16. Bergmeyer, H.U., Bernt,   (1974). Determination of glucose with  glucose  oxidase  and  peroxidase.  In: HU Bergmeyer (Ed.), Methods of enzymatic analysis, VerlagChemie-Academic Press, New York: 1205-15.
17. Cheesbrough, M. (2010).Measurement of serum alpha amylase activity: in District Laboratory Practice in Tropical Countries Part 1, 4^th edition, Cambridge University press, Pp. 360-62.
18. Al-Dahhan, N.A.A. (2015). The Association between Liver Enzymes and Duration of Type2 Diabetes Mellitus. Internationa                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  l Journal of Research Studies in Biosciences; 3(11):60-6.
19. Wohlfahrt-veje et al (2014). Body fat throughout Children: agreement of BMI, waist circumference, skin folds with dual x-ray absorptiometry. j. Clin. Nutr, 68 (6), pp 664-70.
20. WHO (2016). Global report on diabetes. World Health Organization. http://www.who.int/iris/handle/10665/204871.
21. Gray N, Picone G, Sloan F and Yashkin A. The relationship between BMI and Onset of Diabetes Mellitus and its Complications (2015).South Med. J.108(1): 20-6. doi: 10.14423/SMJ.000000000000214
22. Ogbodo, E.C., Okafor, C.C., Ogah, H.G.O., Ezeugwunne, I.P., Igwebuobi, F., Okezie, A.O., Agada, U.N., Amah, A.K., Odumodu, I.O. (2019). Thyroid Hormone Profiling and Enzymatic Antioxidant Status in Diagnosis and Management of Type-Ii-Diabetes Mellitus: A Review of Literature. World Journal of Pharmaceutical and Life Sciences; 5(12):6-21.
23. Kasuga M (2006). Insulin resistance and Pancreatic β cell failure. J. Clin Invest, 118 (7): 1756- 60.
24. Al-Goblan, A.S., Al-Alfi, M.A., Khan, M.Z. (2014). Mechanism linking diabetes mellitus and obesity. Diabetes, metabolic syndrome and obesity: targets and therapy; 7:587-91.
25. Ghimire, S., Shakya, S., Shakya, J., Acharya, P., Pardhe, B.D. (2018). Abnormal Liver parameters among Individuals with Type 2 Diabetes Mellitus Nepalese Population. Biochemistry and Pharmacology (Los Angel); 7: 243.
26. Mahesh, B.M., Chandrashekhar, M.I., Mamatha, T.M., Satish, K.W., Deepak, S.H. (2016). Evaluation of Biochemical Markers Serum Amylase and Serum Lipase for the Assessment of Pancreatic Exocrine Function in Diabetes Mellitus. Journal of Clinical and Diagnostic Research; 10(11): BC01-BC04.
27. Subedi, S., Shrestha, L., Gurung, K., Adhikari, B., Dhakal, G. (2020). Status of Serum Amylase in type 2 Diabetic Patients in Pokhara, Nepal. Journal of Hematology and Allied Sciences; 10(2):63-7.
28. Alzahrani, S.H., Baig, M., Bashawi, J.I., Aashi, M., Shaibi, F.K., Alqami, D.A (2019). Prevalence and Association of Elevated Liver Transaminases in Type 2 Diabetes Mellitus Patients in Jeddah, Saudi Arabia. Cureus; 11(7): e5166.