Free Radical Scavenging Potential of Cobalt Nanoparticles Synthesized from Ipomoea Batatas Leaves Extract

Vol 9 No 4 (2026): Volume 09 Issue 04 April 2026

Article Date Published : 29 April 2026 | Page No.: 2148-2160 | Google Scholar | Crossref doi for this article

Abstract :

Synthesizing nanoparticles with high antioxidant capacity through green routes is critical for creating biocompatible antioxidants. This present study investigated the free-radical scavenging potential of cobalt nanoparticles (CoNPs) synthesized from Ipomoea batatas leaf extract. Air-dried leaf powder was macerated separately with distilled water and ethanol for 48 h, respectively. The filtrates were lyophilized to give aqueous and ethanol extracts. Total phenolic, flavonoid and tannin contents were quantified on both extracts, followed by DPPH scavenging assay. The ethanol extract that exhibited higher activity was utilized in the synthesis of CoNPs. The NPs were characterized via UV, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM-EDX) analysis. The results showed that the ethanol extract demonstrated higher DPPH scavenging activity (IC50 66.49 µg/ml) than aqueous extract (IC50: 641.35 µg/ml) and CoNPs (IC50: 175.18 µg/ml). The CoNPs also competed favorably with ascorbic acid for ferric reducing potential. UV absorption peak was observed at 220 nm, corresponding to the surface plasmon resonance of CoNPs. The FT-IR showed characteristic peaks at <800 cm⁻¹ which is characteristic of cobalt oxide bond. The XRD and SEM-EDX analyses showed that the CoNPs were nanocrystalline, spherical, and well-dispersed with an average size of 17–22 nm. The study concludes that the synthesized CoNPs exhibited significant in vitro antioxidant potential which could be further explored for in vivo antioxidant potential.

Keywords :

Characterization., cobalt nanoparticle, DPPH, free radical, Ipomoea batatas

References :

  1. Shahidi, F. (2012) Nutraceuticals, functional foods and dietary supplements in health and disease. Food Drug Anal. 20(1): 226-230 https://doi.org/10.38212/2224-6614.2144
  2. Farooq, M.U., Mumtaz, M.W., Mukhtar, H., Rashid, U, Akhtar, M.T., Raza, S.A. and Muhammad Nadeem, M. (2020) UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanol leaf extract of Butea monosperma. Scientific Reports: Nature Research.10:3530 https://doi.org/10.1038/s41598-020-60076-5
  3. Halliwell, B. (2011) Free radicals and antioxidants – quo vadis? Trends Pharmacol. Sci. 32(3):125 – 130 DOI: 1016/j.tips.2010.12.002
  4. Sudha, G., Sangeetha Priya, M., Indhu, S R. and Vadivukkarasi, S. (2011) In vitro free radical scavenging activity of raw pepino fruit (Solanum muricatum aiton). Int J Curr Pharm Res: 137-140.
  5. Rahman, T., Hosen, I., Towhidul-Islam, M. M. and Shekbar, H.U. (2012) Oxidative stress and human health. Advances in Bioscience and Biotechnology. 3(7): 997-1019 DOI: 4236/abb.2012.327123
  6. Ye, Z.W., Zhang, J., Townsend, D.M. and Tew, K.D. (2015) Oxidative stress, redox regulation and diseases of cellular differentiation. Biophys. Acta 1850(8): 1607 – 1621 DOI: 10.1016/j.bbagen.2014.11.010
  7. Agarwal, A., Aponte-Mellado, A., Premkumar, B.J., Shaman, A. and Gupta, S. (2012). The effects of oxidative stress on female reproduction: a review. Reproductive Biology and Endocrinology. 10:49. doi: 1186/1477-7827-10-49
  8. Otitolaiye, C.A., Makusidi, A.M., Ndodo, N.D., Labbo, A.M. and Bashiru, I. (2019) Role of oxidative stress in glycated hemoglobin among chronic kidney disease (CKD) patients in Sokoto. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). 18(5): 69-75 DOI:9790/0853-1805066975
  9. Cardoso, V.F., Francesko, A., Ribeiro, I., Bañobre‐López, M., Martins, P. and Lanceros‐Mendez, S. (2018) Advances in magnetic nanoparticles for biomedical applications. Adv Healthcare Mater. 7(5):1700845. doi: 10.1002/adhm.201700845
  10. Azharuddin, M., Zhu, G.H., Das, D., Ozgur, E., Uzun, L. and Turner, A.P. (2019) A repertoire of biomedical applications of noble metal nanoparticles. Chem Commun.55(49): 6964– Doi: 10.1039/C9CC01741K
  11. Das, D. and Saikia, B.J. (2023) Synthesis, characterization and biological applications of cobalt oxide (Co3O4) nanoparticles. Chemical Physics Impact 6:100137. org/10.1016/j.chphi.2022.100137
  12. Dhuper, S., Panda, D. and Nayak, P.L. (2012) Green synthesis and characterization of zero valent iron nanoparticles from the leaf extract of Mangifera indica. Nano Trends: J Nanotech App 13(2):16–22.
  13. Ahmed, K., Tariq, I., Siddiqui, S.U. and Mudassir, M. (2016) Green synthesis of cobalt nanoparticles by using methanol extract of plant leaf as reducing agent. Pure and applied biology. 5(3): 453-457 http://dx.doi.org/10.19045/bspab.2016.50058
  14. Oyeniyi, Y.J. and Mumuni, A.M. (2021) Formulation development of an herbal hand sanitizer containing Moringa oleifera silver nanoparticles. Brazilian Journal of Technology, Curitiba. 4(1):36-49 DOI:10.38152/bjtv4n1-003
  15. Parveen, S., Misra, R. and Sahoo, S.K. (2012) Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging. Nanomedicine: Nano Technol. Biol. Med. 8(2):147-166 doi:10.1016/j.nano.2011.05.016
  16. Smith, D.M., Simon, J.K. and Baker Jr, J.R. (2013) Applications of nanotechnology for immunology. Nat Rev Immunol. 13(8):592– DOI: 10.1038/nri3488
  17. Waris, A., Din, M., Ali, A., Afridi, S., Baset, A., Khan, A.U. and Ali, M. (2021) Green fabrication of Co and Co3O4 nanoparticles and their biomedical applications: A review. Open Life Sciences. 16: 14–30 https://doi.org/10.1515/biol-2021-0003
  18. Ansari, S.M., Bhor, R.D., Pai, K.R., Sen, D., Mazunder, S., Ghosh, K., Kolekar, Y.D. and Ramana, I.V. (2017) Cobalt nanoparticles for biomedical applications: Facile synthesis, physiochemical characterization, cytotoxicity behavior and biocompatibility. S0169433217306530.https://www.sciencedirect.com/science/article/pii/S0169433217306530
  19. Adekunle, A.S., Oyekunle, J.A.O., Durosinmi, L.M., Oluwafemi, O.S., Olayanju, D.S., Akinola, A.S., Obisesan, O.R., Akinyele, O.F., and Ajayeoba, T.A. (2020) Potential of cobalt and cobalt oxide nanoparticles as nanocatalyst towards dyes degradation in wastewater. Nano-Struct, Nano-Objects 21: 100405.
  20. Eleraky, N.E., Allam, A., Hassan, S.B. and Omar, M.M. (2020) Nanomedicine fight against antibacterial resistance: an overview of the recent pharmaceutical innovations. Pharmaceutics. 12(2):142. doi: 3390/pharmaceutics12020142
  21. Padigya, P.R.M., Koyyati, R. and Kudle, K.R. (2016) Evaluation of Antibacterial and cytotoxic activity of green synthesized cobalt nanoparticles using Raphanus sativus var. longipinnatus leaf extract. J. PharmTech Res. 9(3):466-472.
  22. Malathy, D. and Revathi, M. (2021) Green synthesis of cobalt nanoparticles using ethanolic extract of Cadiospermum halicacebium characterization and its anticancer applications. Research Journal of Pharmacy and Technology. 14(3):1530-1534. DOI: 5958/0974-360X.2021.00271.7
  23. Abidin, P.E. (2004) Sweet potato breeding for northeastern Uganda: Farmer varieties, farmer-participatory selection, and stability of performance (PhD Thesis). The Netherlands: Wageningen University. Pp 152 ISBN 90-8504-033-7.
  24. Escobar-Puentes, A.A., Palomo, I., Rodríguez, L., Fuentes, E., Villegas-Ochoa, M.A., González-Aguilar, G.A., Olivas-Aguirre, F.J. and Wall-Medrano, A. (2022) Sweet Potato (Ipomoea batatas ) Phenotypes: From Agroindustry to Health Effects. Foods. 11(7): 1058 doi: 10.3390/foods11071058
  25. Alam, M.A. (2021) A comprehensive review of sweet potato (Ipomoea batatas [L.] Lam): Revisiting the associated health benefits. Trends in Food Science & Technology. 115: 512-529, ISSN 0924-2244 https://doi.org/10.1016/j.tifs.2021.07.001.
  26. Pochapski M.T., Fosquiera E.C,, Esmerino L.A., dos Santos E.B., Farago P.V., Santos F.A., and Groppo F.C. (2011) Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves’ extract from Ipomoea batatas (L.) Lam. Pharmacogn Mag. 7(26):165–170 doi: 4103/0973-1296.80682
  27. Mohanraj, R. and Sivasankar, S. (2014) Sweet Potato (Ipomoea batatas [L.] Lam) – A valuable medicinal food: A review. Med. Food. 17(7):733–741 doi: 10.1089/jmf.2013.2818
  28. Meira, M., Da Silva, E.P., David, J.M., David J.P. (2012) Review of the genus Ipomoea: Traditional uses, chemistry, and biological activities. Bras. Farmacogn. 22: 682–713 https://doi.org/10.1590/S0102-695X2012005000025
  29. Wang, S., Nie, S. and Zhu, F. (2016) Chemical constituents and health effects of sweet potato. Food Res. Int. 89(1): 90–116 DOI: 1016/j.foodres.2016.08.032
  30. Dwivedi AD, Gopal K. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids Surf A. 2010 Oct 20;369(1–3):27–
  31. Otitolaiye, I., Omonkhua, A., Oriakhi, K., Okello, E., Onoagbe, I. and Okonofua, F. (2023) Phytochemical analysis and in vitro antioxidant potential of aqueous and ethanol extracts of Irvingia gabonensis stem bark. Pharmacog Res. 15(2):363-72. DOI:5530/pres.15.2.039
  32. Akinpelu B.A., Godwin A., Gbadegesin T., Ajakaye N., Omotosho S.E., Azeez S.O., Oziegbe M. and Oyedapo O.O. (2019) Comparative studies on the anti-inflammatory, antioxidant and anti-mutagenic activities of Crassocephalum crepidioides (Bent) leaf cold and hot water extracts. Asian Food Science Journal 9(1): 1-12 doi:10.9734/AFSJ/2019/v9i130000
  33. Diniyah, N. and Lee, S.-H. (2020) Phenolic composition and antioxidant potential of legumes – a review. Agroteknologi, 14(1): 91–102 doi: 10.19184/j-agt.v14i01.17965.
  34. Rice-Evans, C (2004). Flavonoids and Isoflavones: absorption, metabolism and bioactivity. Free Rad Biol 36(7): 827-828. DOI: 1016/j.freeradbiomed.2003.12.012
  35. Ou B., Huang D., Hampsch-Woodill M., Flanagan J.A. and Deemer E.K. (2002) Analysis of antioxidant activities of common vegetables employing Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP) assays: A comparative study. J Agric Food Chem. 50(11):3122-8. doi: 10.1021/jf0116606, PMID 12009973.
  36. Huang D., Ou B. and Prior R. L (2005). The chemistry behind antioxidant capacity assays. J Agric Food Chem. 53(6):1841-56. doi: 10.1021/jf030723c, PMID 15769103.
  37. Adebiyi O.E., Olayemi F.O., Ning-Hua T. and Guang-Zhi Z. (2017) In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni Suef Univ J Basic Appl Sci. 6(1):10-4. doi: 10.1016/j.bjbas.201 6.12.003.
  38. Izadiyan, Z., Shameli, K., Miyake, M., Hara, H., Mohamad, S.E.B., Kalantari, K., Taib, S.H.M. and Rasouli, E. (2020) Cytotoxicity assay of plant-mediated synthesized iron oxide nanoparticles using Juglans regia green husk extract. J. Chem. 13: 2011–2023. Doi: 10.1016/J.ARABJC.2018.02.019
  39. Wong, I., Li, H., Cheng, K. and Chen, F. (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97: 705–711. https://doi.org/10.1016/j.foodchem.2005.05.049
  40. Hongyan, X, L., Qiang, Z., Junyangli, J. D., Xiujian, C., Jun, T. C. X. (2014). Preparation Method of Co3O4 Nanoparticles Using Degreasing Cotton and Their Electrochemical Performance in Super Capacitor. Journal of Nanoparticles 4(1):1-9. http://dx.doi.org/10.1155/2014/723057
  41. Wadekar, K.F., Nemade, K. R., and Waghuley, S. A. (2017). Chemical Synthesis of Cobalt Oxide Nanoparticles Using Co-Precipitation Method. Research Journal of Chemical Sciences. 7(1): 53-55
  42. Danbature, W. L., Isyaka M. Sani, Abdullahi M Abdullahi, Gambo, A. A. and Abdulmalik, S. S. (2023) UV-VIS, FTIR and XRD characterization of synthesized magnetic cobalt (Co3O4) nanoparticle used in catalytic decomposition of hydrogen peroxide. FUDMA Journal of Sciences (FJS) Vol. 7 No. 6, December 2023, pp 222 -227 DOI: https://doi.org/10.33003/fjs-2023-0706-2105
  43. Suman, T. Y., Elumalai, D., Kaleena, P. K., and S. R. R. Rajasreea (2013) GC–MS analysis of bioactive components and synthesis of silver nanoparticle using Ammannia accifera aerial extract and its larvicidal activity against malaria and filariasis vectors, Industrial Crops and Products. 47: 239–245. https://doi.org/10.1016/j.indcrop.2013.03.010
  44. Parethe, G. T., Rajesh, P., Nathiya, P., Balaji, M. and Kavica, S. (2023) Green synthesis of cobalt oxide nanoparticles from Clitoria ternatea flower extracts its characterization and biological activities. World Journal of Advanced Research and Reviews. 19(01): 001–009 org/10.30574/wjarr.2023.19.1.1301
  45. Du, H., Jiao, L., Wang, Q. Yang, J., Guo, L., Si, Y., Wang, Y. and Yuan, H. (2013) Facile carbonaceous microsphere templated synthesis of Co3O4 hollow spheres and their electrochemical performance in supercapacitors,” Nano Research. 6(2): 87-98. https://doi.org/10.1007/s12274-012-0283-5
  46. Wang, X., Yu, L, Wu, X. Yuan, F, Guo, Y, Ma, Y. and Yao, J. (2009) Synthesis of single-crystalline Co3O4 octahedral cages with tunable surface aperture and their lithium storage properties. Journal of Physical Chemistry C. 113(5):15553 – 15558. DOI:1021/JP904652M

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Free Radical Scavenging Potential of Cobalt Nanoparticles Synthesized from Ipomoea Batatas Leaves Extract. Catherine Otitolaiye, Bashiru Ibrahim, Mustapha Muhammad, Umar Abdussalam, 9(4), 2148-2160. Retrieved from https://ijcsrr.org/single-view/?id=26063&pid=25854

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