Abstract :

Phytochemical research has attracted the attention of researchers in the field of biomedical and biotechnological research and explored a new era of drug discovery. Focus of these studies is to minimize or overcome the side effects of synthetic drugs.  It aims to find out the equivalent natural molecules that can replace the drugs having harmful side effects on human health.

Advances in the organic and analytical chemistry during last century offered new vision towards the phytochemical research. Investigations of various molecules through different biochemical and biophysical methods can easily reveals the structural and functional properties of the compounds and have opened the new avenues in natural drug discovery. These new techniques are more competent to isolate, characterize and analyze the active principles from these medicinal plants and other natural resources. Thus, the phytochemical research has proved to have a pivotal role in drug discovery.

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Keywords :

Antioxidants., Oxygen Paradox, RNS., ROS

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References :

1. L, M. ((1996). Antioxidant Flavonoids: Structure,Function & Clinical Usages;. Alternative Medicine Review;, 103-111.
2. Adwas, A., Elsayed, A., Azab, A., & Quwaydir, F. (2019). Oxidative stress and antioxidant mechanisms in human body. . J. Appl. Biotechnol. Bioeng., 6, 43–47 .
3. , N. (2003). Vitamin C in human health and disease is still a mystery ? An overview. . Nutr. J. , 2, 1-10.
4. , M. (1996). Antioxidant Flavonoids: Structure, Function and Clinical Usage.. . Alt. Med. Rev, 1, 103-111.
5. Andre C., e. a. (2010). “Analytical strategies to evaluate antioxidants in food: a review” . Trends in Food Science and Technology , 21, 229-246.
6. Bashir, A. R. (1995). “Altering kinetmechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase.”. Biochem J, 312(2), 527-533.
7. Benito, P., Lanza, A., Sen, A., Matellano, L., Gómez, A., Martínez, M., & Galindez, J. (2000). Effects of Some Iridoids from Plant Origin on Arachidonic Acid Metabolism in Cellular Systems. Planta Med, 66, 324–328.
8. Black, A. T. (2008)). “Increased oxidative stress and antioxidant expression in mouse keratinocytes following exposure to paraquat. Toxicol Appl Pharmacol, 231(3), 384-392.
9. Busse, W. (1998). Leukotrienes and inflammation. . Am. J. Respir. Crit. Care Med., 157, S210–S213.
10. Divya, T., Latha, P., Usha, K., Anuja, G., Suja, S., Shyamal, S., . . . Rajasekharan, S. (2009). Anti-inflammatory, analgesic and anti-lipid peroxidative properties of Wattakaka volubilis (Linn. f.) Stapf. Indian J. Nat. Prod. Resour, 8, 137–141.
11. Dorge, W. (2002 ). Free Radical in the Physiological Control of Cell Function . physiology Rev, vol 82, 30-55.
12. Dugan L.L., C. D. (1999). Basic neurochemistry. Molecular,Cellular & medical aspects 6th Edition . Philadelphia : Lippincott Raven.
13. Dugan L.L., C. D. (1999). .Basic neurochemistry. Molecular,Cellular & medical aspects 6th Edition. Philadelphia : Lippincott Raven.
14. Sibal Pala, K. T. (2007). Free radicals : Our enemies or Friends? . Adv.in Mol. Biol , 1, 63-69.
15. Sibal Pala, K. T. (2007). Free radicals : Our enemies or Friends? . Adv.in Mol. Biol, 1, 63-69.
16. Sibal Pala, K. T. (2007). Free radicals : Our enemies or Friends? . Adv.in Mol. Biol, 1, 63-69.
17. Florian R. Greten, S. I. (July 16, 2019). Inflammation and Cancer: Triggers, Mechanisms, and Consequences, . Immunity , 51, 27-41.
18. C.Brown, V. (2001). Nitric oxide,mitochondria and cell Death . IUBMB Life (52), 189-195.
19. Loschen, B. F. (1971). Chance respiratory chain linked H2O2 production in Pigeon heart mitochondria. FEBS Letter 18, 261-263.
20. Loschen, B. F. (1971). Chance respiratory chain linked H2O2 production in Pigeon heart mitochondria. FEBS Letter, 18, 261-263 .
21. Gupta, S. K. (2010). International Research Journal of Plant Science, Vol. 1(5), 118-125.
22. Halliwell B, G. J. (1995). The definition and measurement of antioxidants in biological systems. ;. Free Radic Biol Med, 18, 125-6.
23. Halliwell B, G. J. (1995). The defination and measurement of antioxidant in biological systems. Free Radical Biology & Medicine, 126-6.
24. , M. L. (2000). Oxyradicals and DNA damage. Carcinogenesis( 21), 361-370.
25. E, F. R. (1990). Hydroxy free radical damage to DNAIn membrane Lipid Oxidation Vol 1 . Boca Ration Florida: Vigo Pelfrey ,C,CRC press.
26. JA, L. V. (2000). OH radicals and oxidizing products in the gamma radiolysis of water. :. Radiat. Res., 153, 196-200.
27. JF, W. (1995). Radiation mutagenesis: The initial DNA lesions responsible. Radiation . Res., 142, 362-380.
28. Jo, W., Yang, K., Choi, Y., Jeong, C., Ahn, K., Nam, B., . . . Jeong, M. (2010). In vitro and in vivo anti-inflammatory effects of pegmatite. Mol. Cell. Toxicol., 6, 195–202.
29. K . R. Patil, e. (2019). Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. Int. J. Mol. Sci., 4367, 20, 367.
30. Kalpesh R. Patil, U. B. (n.d.). Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discove.
31. Koh TJ, D. L. ( 2011). Inflammation and wound healing: The role of the macrophage. . Expert Rev Mol Med, 13-23.
32. Kohen R, N. A. (2002). Kohen R,Nyska A. Oxidation of biological systemsOxidation of Biological System : Oxidative stress phenomena, antioxidants, redox reactions,and methods for their quatification. . Toxicol Pathol, 30, 620-630.
33. Kumari, K., Weerakoon, T., Handunnetti, S., Samarasinghe, K., & Suresh, T. (2014). Anti-inflammatory activity of dried flower extracts of Aegle marmelos in Wistar rats. . J. Ethnopharmacol. (151), 1202–1208.
34. (1996). Oxidants, antioxidants and disease preventation, ,. Belgium, International Life Science Institute: Oxford Press , 347-378.
35. Li Y, S. H. (2007). New developments and novel therapeutic perspectives for vitamin C. Critical Review. J. Nutr(137), 2171-2184.
36. M Inoue, E. N. (2003). Mitochondrial 319 generation of reactive oxygen species and its role in aerobic life. Curr. Med. Chem., 10 (320), 2495-2505.
37. MacGlashan, D. (2003). Histamine: A mediator of inflammation . J. Allergy Clin. Immunol., 112, 53.
38. Meister A, A. M. (1983). “Glutathione”. Annu. Rev. Biochem, 52, 711-760.
39. MF, B. (2002). Oxidatively modified proteins in ageing and diseases. . Free Radiat. Biol. Med. , 32:, 797-803.
40. Ndiamaka H Okorie., e. a. (2019). “Antioxidants Properties of Natural and Synthetic Chemical Compounds: Therapeutic Effects on Biological System”. . Acta Scientific Pharmaceutical Sciences, 3.6, 28-42.
41. Newman, D. J. ( 2016). .Natural Products as Sources of New Drugs . J. Nat. Prod. , 79 (3), 629–661.
42. Ogbonna, J. K. (2012). Different approaches to formulation of herbal extracts/ phytopharmaceuticals/bioactive phytochstituents – a review. Int. J. Pharm. Sci. Rev. Res, 16(1), 1–8.
43. PA, R. (1994). Free radicals in biology: Oxidative stress and the effects of ionizing radiation. . Int. J. Radiat. Biol., 65, 27-33.
44. Packer L, W. S. (2001). Molecular aspects of alphatocotrienol antioxidant action and cell signalling. . J. Nutr, 131(2), 369S–373S.
45. Perry, J. J. (2010). “The structural biochemistry of the superoxide dismutases.”1: . Biochim Biophys Acta , 804(2), 245-262.
46. Peter Libby, M. ( December 2007(II)). Inflammatory Mechanisms: The Molecular Basis of Inflammation and Disease . Nutrition Reviews, Vol. 65( No. 1), S140–S146.
47. peter Mc clintock. (March 2015). Review of ” The Origin of life on Earth”. Contemporary Physics 57(1), 1-3.
48. , J. (1995). The integrated antioxidant system. . . Nutr Res, 15, 755-766. .
49. Rao, G. M. (2000). Role of antioxidant enzymes in brain tumours. Clin Chim Acta 296(1-2), 203-212.
50. Reiter RJ, C. R. (1997). . Melatonin in relation to cellular antioxidative defense mechanisms. Horm. Metab. Res, 29((8)), 363- 372.
51. Schreck R, B. D. (1991). A Role for oxygen radical as second messengers . Trends Cell Biol, 1, 39-42.
52. Sies, H. (1997). Oxidative stress: oxidants and antioxidants. Exp.Physiol, (2)(82), 291–295.
53. Suntres, Z. E. (2002). “Role of antioxidants in paraquat toxicity.” . Toxicology , 180(1), 65-77.
54. Tan DX, M. L. (2000). Significance of melatonin in antioxidative defense system: reactions and products. . Biological signals receptors, , 9(3-4), 137-159.
55. N.Wadekar,C.N.Khobragade. (2016). Oxidative Stress and Redox Homeostasi in Biological Cells. SRTM University’s Research Journal of Science, 7-10.
56. Valko M, L. D. (2007). Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry and Cell Biology, 39, 44-84.
57. Valko M, M. H. ( 2005). Metals, toxicity and oxidative stress. Curr. Med. Chem(12), 1161-1208.
58. Weiss JF, L. M. (2009). History and development of radiation protective agents. . Int. J. Radiat. Biol., 85, 539-573.
59. Willcox JK, A. S. (2004). Antioxidants and prevention of chronic disease. . Review. Crit. Rev. Food. Sci. Nutr., 275-295.
60. , L. (2011). “Antioxidant in biology and medicine: essentials, advances, and clinical applications”. :. Nova Science Publishers USA, 22.