Abstract :

The global livestock sector is facing mounting pressure to adopt sustainable and efficient feed alternatives that can meet the growing demand for animal protein. Among the emerging options, Hermetia illucens (Black Soldier Fly, BSF) oil presents a promising candidate due to its bioactive compound richness and capacity to upcycle organic waste into nutrient-dense biomass. This study investigates the essential and non-essential amino acid composition of BSF oil extracted from larvae (8 days), prepupae (14 days), and pupae (28 days) stages and evaluates its potential as a feed additive in broiler production. High-Performance Liquid Chromatography (HPLC) revealed significant variations across developmental phases, with larval oil exhibiting the highest lysine (4,467.56 mg/100g) and glutamic acid (7,564.45 mg/100g), while pupal oil was enriched in leucine (5,670.76 mg/100g) and tryptophan (4,356.79 mg/100g). The dynamic amino acid profile supports the inclusion of BSF oil as a stage-specific supplement in poultry feed, offering both nutritional and economic advantages. Findings from this study contribute to the growing body of knowledge on insect-based feed ingredients and provide critical insight for formulating phase-targeted broiler diets with optimized functional performance. However, essential amino acids such as leucine, lysine, and tryptophan remained stable or even increased. Among all stages, larval oil exhibited the richest amino acid composition, suggesting its suitability for use in high-performance starter feeds for broilers.

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Keywords :

amino acid, Black Soldier Fly, broiler, feed additive, oil

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References :

1. Azizah, F. L., Sjofjan, O., & Widodo, E. (2024). Assessing the impact of black soldier fly oil (Hermetia illucens) from various phases as feed additive on the growth performance and histomorphology of broiler chickens. Advances in Animal and Veterinary Sciences, 12(3), 509–514.
2. Barragan-Fonseca, K. B., Dicke, M., & van Loon, J. J. A. (2021). Nutritional value of the black soldier fly (Hermetia illucens) and its suitability as animal feed – A review. Journal of Insects as Food and Feed, 7(5), 761–780.
3. Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., Dossena, A., & Sforza, S. (2018). Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin. Food Research International, 105, 812–820.
4. Gadzama, I. (2025). Black soldier fly and circular agriculture: Nutritional potential and sustainability implications (FAO Technical Report on Insect Protein in Agroecology). Rome: Food and Agriculture Organization of the United Nations.
5. Hale, O. M. (1973). Dried Hermetia illucens larvae (Diptera: Stratiomyidae) as a feed additive for poultry. Journal of the Georgia Entomological Society, 8, 16–20.
6. Huang, J., Wang, Q., Chen, Q., Ye, X., Liu, T., & Xie, J. (2025). The addition of Hermetia illucens to feed: Influence on nutritional composition, protein digestion characteristics, and antioxidant activity. Foods, 14(7), 1140.
7. Kim, Y. H., Kim, T. S., Lee, S. H., Park, S. H., & Kwak, H. S. (2021). Effect of dietary black soldier fly oil on growth performance, blood characteristics and meat quality in broiler chickens. Journal of Poultry Science, 58(4), 222–229.
8. Lemme, A., & Klüber, P. (2024). Rethinking amino acid nutrition of black soldier fly larvae (Hermetia illucens) based on insights from an amino acid reduction trial. Insects, 15(11), 862.
9. Liu, X., Chen, X., Wang, H., Yang, Q., ur Rehman, K., Li, W., Cai, M., Li, Q., Mazza, L., Zhang, J., Yu, Z., Zheng, L., & Tomberlin, J. K. (2019). Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly. Journal of Insects as Food and Feed, 5(3), 289–299.
10. Maulana, M., Rahmawati, F., & Pangestu, D. R. (2025). Pengaruh pemberian larva Hermetia illucens terhadap efisiensi pakan dan performa broiler. Tropical Wetland Journal, 11(1), 22–32.
11. Opoku, E., Renna, M., Schøn, N., & Gai, F. (2023). Optimal dietary protein content and essential amino acid limitation in larvae of the black soldier fly (Hermetia illucens). Journal of Insects as Food and Feed, Advance online publication.
12. Permana, S. A., Susilo, F. X. C., & Trisyono, Y. A. (2024). Utilization of Hermetia illucens meal in broiler diets: Growth response and gut histology. Asian Journal of Poultry Science, 18(2), 87–96.
13. Saidani, S. A., Al-Fadhlan, H. M., & Rahman, N. (2025). Effect of dietary inclusion of black soldier fly (Hermetia illucens) larvae on cecal microbiota and blood profile of broiler chickens. Veterinary and Animal Science, 21, 100605.
14. Widiyastuti, T., Nurhidayati, N., Lestari, D. A., Farihatun, F., & Susana, I. W. R. (2024). Nutrient profile and digestive enzyme activity of Hermetia illucens larvae reared on different substrates. Online Journal of Animal and Feed Research, 14(5), 309–320.
15. Zulkifli, N. F. N. M., Seok-Kian, A. Y., Seng, L. L., Mustafa, S., Kim, Y.-S., & Shapawi, R. (2022). Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods. PLOS ONE, 17(2), e0263924.