Abstract :

Silver nanoparticles had been synthesized through the bioreduction process using daruju (Acanthus ilicifolius) leaf extract as a bioreductant. The variation of the formula used is a mixture of daruju leaf extract with a 0.01 M AgNO₃ solution with volume ratios of 2:1, 1:1, 1:2, and 1:3. The research results show that UV-Visible spectrophotometry confirmed that a volume ratio of 1:1 is the optimum formula with an absorption value of 2.346 at the maximum wavelength 440 nm. Synthesis of silver nanoparticles with daruju leaf extract has a strong inhibition zone about 10.42 mm. Proving that the synthesis of silver nanoparticles with extract daruju leaf can be used as an antibacterial agent against E.coli bacteria.

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Keywords :

Bioreductor, Daruju Leaf extract, E. coli, Silver nanoparticle, Synthesis nanoparticle.

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References :

1. Chugh, V. S. Viswamalya, and B. Das, “Green synthesis of silver nanoparticles with algae and the importance of capping agents in the process,” J. Genet. Eng. Biotechnol., vol. 19, no. 1, p. 126, 2021, doi: 10.1186/s43141-021-00228-w.
2. J. Cameron, F. Hosseinian, and W. G. Willmore, “A current overview of the biological and cellular effects of nanosilver,” Int. J. Mol. Sci., vol. 19, no. 7, pp. 1–40, 2018, doi: 10.3390/ijms19072030.
3. G. Kaiser et al., “Nanosilver: An Old Antibacterial Agent with Great Promise in the Fight against Antibiotic Resistance,” Antibiotics, vol. 12, no. 8, 2023, doi: 10.3390/antibiotics12081264.
4. F. Aritonang, H. Koleangan, and A. D. Wuntu, “Synthesis of silver nanoparticles using aqueous extract of medicinal plants’ (impatiens balsamina and lantana camara) fresh leaves and analysis of antimicrobial activity,” Int. J. Microbiol.,vol. 2019, 2019, doi: 10.1155/2019/8642303.

5. Suhatri, P. Ardiningsih, Wiiyantoro ari, and et al, “Senyawa sitotoksik dari fraksi diklorometana daun daruju (Acanthus ilicifolius Linn.) terhadap sel hela,” J. Kim. Khatulistiwa, vol. 7, no. 2, pp. 75–81, 2018, [Online]. Available:https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/25191

6. Latief, L. Marlinda, I. L. Tarigan, and R. Dyah Puspitasari, “Pengolahan Daun Jeruju Menjadi Produk Pangan Fungsional di Kelurahan Tanjung Solok,” J. Pengabdi. Masy. Indones., vol. 4, no. 1, pp. 49–53, 2024, doi:10.52436/1.jpmi.1951.

7. Liu et al., “Structure and genetics of escherichia coli O antigens,” FEMS Microbiol. Rev., vol. 44, no. 6, pp. 655–683,2020, doi: 10.1093/femsre/fuz028.

8. R. H. y w li, “Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe β -hemolitik dan Non-hemolitik pada Ikan Nila,” vol. 12, no. 2, pp. 152–164, 2011.

9. Setiani and S. Suyatno, “Synthesis and Characterization of Copper Nanoparticles with Bioreductor Carica Dieng (Carica pubescens) Seed Extract,” J. Pijar Mipa, vol. 19, no. 1, pp. 150–155, 2024, doi: 10.29303/jpm.v19i1.6124.
10. Javani and S. Sutoyo, “Indonesian Journal of Chemical Science Potential of the Mangosteen Peel Extract (Garnicia mangostana L.) as a Bioreductor in the Synthesis of Copper Nanoparticles,” Indones. J. Chem. Sci., vol. 12, no. 3, 2023,[Online]. Available: http://journal.unnes.ac.id/sju/index.php/ijcs

11. I. Achrifa and S. Suyatno, “Phytochemical Screening and Nanoherbs Synthesis of Ethanol Extract of the Butterfly Pea Flower (Clitoria ternatea L.) with its Characterization,” J. Pijar Mipa, vol. 19, no. 1, pp. 156–161, 2024, doi:10.29303/jpm.v19i1.6125.

12. Sutoyo, Tukiran, and S. Khotijah, “Antioxydant activity of the silver nanoparticles (AgNPs) synthesized using Nephrolepisradicans extract as bioreductor,” J. Phys. Conf. Ser., vol. 1747, no. 1, 2021, doi: 10.1088/1742-6596/1747/1/012038.

13. Pehino, F. Fatimawali, and E. J. Suoth, “UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI BUAH DUKU Lansium
14. domesticum TERHADAP BAKTERI STAPHYLOCOCUS AUREUS DAN ESCHERICHIA COLI,” Pharmacon, vol. 10, 2, p. 818, 2021, doi: 10.35799/pha.10.2021.34030.
15. A. Wibowo, D. N. Sari, A. Jayuska, and P. Ardiningsih, “KOMPOSISI KIMIA DAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN KAYU PUTIH (Melaleuca cajuputi) DARI KOTA SINGKAWANG,” Biopropal Ind., vol. 12, no. 1, p. 1, 2021, doi: 10.36974/jbi.v12i1.6509.

16. S. Nurhayati, N. Yahdiyani, and A. Hidayatulloh, “Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram,” J. Teknol. Has. Peternak., vol. 1, no. 2, p. 41, Oct. 2020, doi: 10.24198/jthp.v1i2.27537.

17. Y. Ayen, Rahmawati, and Mukarlina, “Aktivitas Antibakteri Ekstrak Metanol Daun Sembung Rambat (Mikania micrantha H.B.K) Terhadap Pertumbuhan Bakteri Bacillus cereus IHB B 379 dan Shigella flexneri,” J. Protobiont, vol. 10, no. 2, pp. 123–129, 2017.

18. Sutoyo et al., “Synthesis of Nanoherbal from Ethanol Extract of Indonesian Fern Selaginella plana and Antibacterial Activity Assay,” Trop. J. Nat. Prod. Res., vol. 6, no. 1, pp. 44–49, 2022, doi: 10.26538/tjnpr/v6i1.9.
19. D. Pita Rengga, W. P. Hapsari, and D. W. Ardianto, “sintesis nanopartikel tembaga dari larutan CuNO3 menggunakan ekstrak cengkeh (Syzygium aromaticum),” J. Rekayasa Kim. Lingkung., vol. 12, no. 1, pp. 15–21, 2017, doi: 10.23955/rkl.v12i1.5197.

20. P. B. Santoso, E. Puspitasari, and D. R. P, “Uji Efektivitas Daya Hambat Ekstrak Madu terhadap Pertumbuhan Salmonella typhi dengan Metode Difusi Cakram,” STIKes Insa. Cendekia Med. Jombang, vol. 1, no. 1, pp. 1–10, 2020.