Abstract :
Nanosilver is a nanoparticle known for its good antifungal ability. This study aims to determine the synthesis process and characterization of nanosilver produced and the antifungal activity of nanosilver in body lotion preparations. Body lotion in this study was made using a body lotion base which includes stearic acid, cetyl alcohol, TEA, glycerin, olive oil and distilled water. F1, F2, and F3 have nanosilver content of 10%, 15% and 20%, respectively. F4, as the positive control, contained methylparaben and F5 as a negative control. The body lotion produced in this study has a colour, aroma and texture that panellists prefer. The body lotion produced does not irritate the skin, is homogeneous, has good spreadability and has a pH value of 7. Nanosilver in this study was synthesized using a chemical reduction method with sodium citrate as a reducer and stabilizer. The synthesized nanosilver was characterized using a Transmission electron Microscope (TEM) and UV-Vis spectrophotometer to determine the nanosilver’s shape, size and stability. The nanosilver produced has a round shape with an average particle diameter of 34.793 nm and has good stability because it has a wavelength absorption peak that is not large. Namely, at week-0, nanosilver has an absorption peak at a wavelength of 415.20 nm, and week-8 has an absorption peak at a wavelength of 423 nm. The antifungal activity test using the direct microscope count (DMC) method was conducted for eight weeks. Until week 8, it showed that each formulation experienced an increase in fungal hyphae, reaching 34.4% for F1, 21.2% for F2, 19.8% for F3, 16.8% for F4 and 46% for F5. From the results of this study, it can be seen that nanosilver can suppress fungal activity in body lotion preparations.
Keywords :
antifungal, Body lotion, DMC, nanosilver, TEM, UV-vis spectrophotometer.References :
- Tricaesario and R. I. Widayati, “Efektivitas Krim Almond Oil 4% Terhadap Tingkat Kelembapan Kulit.,” Jurnal Kedokteran Diponegoro, vol. 5, no. 4, pp. 599–610, 2016.
- Mistry, “Guidelines for Formulating Anti-Pollution Products,” Cosmetics, vol. 4, no. 4, Dec. 2017, doi: 10.3390/cosmetics4040057.
- Bianti, “Kulit Kering pada Usia Lanjut,” Continuing Medical Education (CDK-245), vol. 43, no. 10, pp. 737–740, 2016, [Online]. Available: http://www.rejuvenateyurskin.co.uk
- Sumbayak and V. E. Diana, “Formulasi Hand Body Lotion Ekstrak Etanol Kulit Buah Semangka (Citrillus vulgaris).,” Jurnal Dunia Farmasi, vol. 2, no. 2, pp. 70–76, 2018.
- Mohiuddin, “Skin Care Creams: Formulation and Use.,” American Journal of Dermatological Research and Reviews, vol. 2, no. 8, pp. 1–45, 2019.
- M. Tjiang et al., “Analisis Kualitatif Dan Kuantitatif Kandungan Paraben Dalam Kosmetik Hand Body Lotion,” Indonesian Journal of Legal and Forensic Sciences, vol. 9, no. 2, pp. 89–96, 2019, [Online]. Available: http://ojs.unud.ac.id/index.php/ijlfs
- Raini, R. S. Handayani, and A. Isnawati, “Gambaran Cemaran Jamur Pada Kosmetik Bedak Bayi dan Bayangan Mata.,” Media Penelitian dan Pengembangan Kesehatan, vol. 14, no. 4, pp. 1–6, 2004.
- Jalestri and T. Taufikurohmah, “Uji Aktivitas Antifungi Nanosilver Dalam Krim Pagi Terhadap Fungi Candida albicans,” UNESA Journal of Chemistry, vol. 7, no. 28, 2016.
- L. Aarflot, “Human Exposures to Parabens in Cosmetics,” Universitiy of Troms, Troms, 2013.
- Pangaribuan, “Efek Samping Kosmetik dan Penanganannya Bagi Kaum Perempuan,” Jurnal Keluarga Sehat Sejahtera, vol. 15, no. 2, pp. 20–28, 2017.
- Nowak, W. Ratajczak-Wrona, M. Górska, and E. Jabłońska, “Parabens and Their Effects on the Endocrine System,” Mol Cell Endocrinol, vol. 474, pp. 238–251, Oct. 2018, doi: 10.1016/j.mce.2018.03.014.
- Du, Z. Hu, W. jie Dong, Y. Wang, S. Wu, and Y. Bai, “Biosynthesis of large-sized silver nanoparticles using Angelica keiskei extract and its antibacterial activity and mechanisms investigation,” Microchemical Journal, vol. 147, pp. 333–338, Jun. 2019, doi: 10.1016/j.microc.2019.03.046.
- Akter et al., “A Systematic Review on Silver Nanoparticles-Induced Cytotoxicity: Physicochemical Properties and Perspectives,” J Adv Res, vol. 9, pp. 1–16, Jan. 2018, doi: 10.1016/j.jare.2017.10.008.
- Sofi, S. Sunitha, M. Ashaq Sofi, S. K. Khadheer Pasha, and D. Choi, “An Overview of Antimicrobial and Anticancer Potential of Silver Nanoparticles,” J King Saud Univ Sci, vol. 34, no. 2, Feb. 2022, doi: 10.1016/j.jksus.2021.101791.
- T. J. Ong and K. L. Nyam, “Evaluation of Silver Nanoparticles in Cosmeceutical and Potential Biosafety Complications,” Saudi J Biol Sci, vol. 29, no. 4, pp. 2085–2094, Apr. 2022, doi: 10.1016/j.sjbs.2022.01.035.
- Al-Otibi et al., “Comparative Study of Antifungal Activity of Two Preparations of Green Silver Nanoparticles from Portulaca oleracea extract,” Saudi J Biol Sci, vol. 29, no. 4, pp. 2772–2781, Apr. 2022, doi: 10.1016/j.sjbs.2021.12.056.
- J. Kim et al., “Antifungal Activity And Mode Of Action Of Silver Nano-Particles On Candida albicans,” BioMetals, vol. 22, no. 2, pp. 235–242, Apr. 2009, doi: 10.1007/s10534-008-9159-2.
- Hadrup, A. K. Sharma, and K. Loeschner, “Toxicity of Silver Ions, Metallic Silver, and Silver Nanoparticle Materials After in Vivo Dermal and Mucosal Surface Exposure: A Review,” Regulatory Toxicology and Pharmacology, vol. 98, pp. 257–267, Oct. 2018, doi: 10.1016/j.yrtph.2018.08.007.
- Rezvani, A. Rafferty, C. McGuinness, and J. Kennedy, “Adverse Effects of Nanosilver on Human Health and the Environment,” Acta Biomater, vol. 94, pp. 145–159, Aug. 2019, doi: 10.1016/j.actbio.2019.05.042.
- E. K. Kraeling et al., “In Vitro Percutaneous Penetration of Silver Nanoparticles in Pig and Human Skin,” Regulatory Toxicology and Pharmacology, vol. 95, pp. 314–322, Jun. 2018, doi: 10.1016/j.yrtph.2018.04.006.
- Vega-Baudrit, S. M. Gamboa, E. R. Rojas, and V. V. Martinez, “Synthesis And Characterization Of Silver Nanoparticles And Their Application As An Antibacterial Agent,” Int J Biosens Bioelectron, vol. 5, no. 5, pp. 166–173, 2019, doi: 10.15406/ijbsbe.2019.05.00172.
- Iravani, H. Korbekandi, S. V Mirmohammadi, and B. Zolfaghari, “Synthesis of Silver Nanoparticles: Chemical, Physical and Biological Methods,” Res Pharm Sci, vol. 9, no. 6, pp. 385–406, 2014.
- Odularu, “Metal Nanoparticles: Thermal Decomposition, Biomedicinal Applications to Cancer Treatment, and Future Perspectives,” Bioinorg Chem Appl, vol. 2018, 2018, doi: 10.1155/2018/9354708.
- Javed, M. Zia, S. Naz, S. O. Aisida, N. ul Ain, and Q. Ao, “Role of Capping Agents in the Application of Nanoparticles in Biomedicine and Environmental Remediation: Recent Trends and Future Prospects,” J Nanobiotechnology, vol. 18, no. 1, Dec. 2020, doi: 10.1186/s12951-020-00704-4.
- Mohaghegh et al., “A Comparative Study of Eco-Friendly Silver Nanoparticles Synthesis Using Prunus Domestica Plum Extract and Sodium Citrate as Reducing Agents,” Advanced Powder Technology, vol. 31, no. 3, pp. 1169–1180, Mar. 2020, doi: 10.1016/j.apt.2019.12.039.
- T. Khatoon, G. V. S. Nageswara Rao, K. M. Mohan, A. Ramanaviciene, and A. Ramanavicius, “Antibacterial And Antifungal Activity Of Silver Nanospheres Synthesized By Tri-Sodium Citrate Assisted Chemical Approach,” Vacuum, vol. 146, pp. 259–265, Dec. 2017, doi: 10.1016/j.vacuum.2017.10.003.
- Wang et al., “Silver Nanoparticles: A Novel Antibacterial Agent For Control of Cronobacter sakazakii.,” J Dairy Sci, vol. 101, no. 12, pp. 10775–10791, Dec. 2018, doi: 10.3168/jds.2018-15258.
- Taufikurohmah, D. Soepardjo, and H. Armadianto, “Synthesis and Characterization of Nanogold-Nanosilver Cluster Diameter Using UV-Visible Instruments and TEM Electron Microscope Transform Instruments,” Advances in Social Science, Education and Humanities Research, vol. 390, pp. 146–151, 2020.
- Saputra, A. Haryono, J. A. Laksmono, and D. M. Hilman Anshari, “Preparasi Koloid Nanosilver dengan Berbagai Jenis Reduktor Sebagai Bahan Anti Bakteri,” Jurnal Sains Materi Indonesia Indonesian Journal of Materials Science, vol. 12, no. 3, pp. 1411–1098, 2011.
- Kristianingsih, F. Sari, and F. Rahayu, “Optimasi Dan Karakterisasi Sediaan Body Lotion Ekstrak Etanol Daun Kelor (Moringa oleiferaL.) Menggunakan Tween 80 Dan Span 80 Sebagai Emulgator,” Jurnal Pharma Bhakta, vol. 2, no. 2, pp. 6–15, 2022.
- Amini, C. D. Hamdin, H. Muliasari, and W. A. Subaidah, “Efektivitas Formula Krim Tabir Surya Berbahan Aktif Ekstrak Etanol Biji Wali (Brucea javanica L. Merr),” Jurnal Kefarmasian Indonesia, pp. 50–58, Feb. 2020, doi: 10.22435/jki.v10i1.2066.
- Nurhaini et al., “Formulasi Gel Ekstrak Etanol Buah Mahkota Dewa (Phaleria Macrocarpa (Scheff.) Boerl) Dengan Variasi Konsentrasi Carbopol Dan Propilenglikol,” CERATA Jurnal Ilmu Farmasi (Journal of Pharmacy Science, vol. 9, no. 2, pp. 68–78, 2018.
- Dominica, D. S. Handayani Prodi, F. Matematika dan Ilmu Pengetahuan Alam, and U. Bengkulu, “Formulasi dan Evaluasi Sediaan Lotion dari Ekstrak Daun Lengkeng (Dimocarpus Longan) sebagai Antioksidan,” Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, vol. 6, no. 1, p. 1, 2019.
- A. Mardikasari, A. Nafisah, T. A. Mallarangeng, W. Ode, S. Zubaydah, and E. Juswita, “Formulasi dan Uji Stabilitas Lotion dari Ekstrak Etanol Daun Jambu Biji (Psidium guajava L.) Sebagai Antioksidan,” Jurnal Farmasi, vol. 3, no. 2, pp. 28–32, 2017.
- A. Subaidah, W. Hajrin, and Y. Juliantoni, “Formulasi dan Evaluasi Sifat Fisik Lotion Ekstrak Etanol Daun Kemuning (Murraya paniculata (L) Jack) dan Daun Lidah Buaya (Aloe vera Linn),” Sasambo Journal of Pharmacy, vol. 1, no. 1, pp. 12–16, 2020, [Online]. Available: http://jffk.unram.ac.id/index.php/ssp/index
- Sanjaya, H. Nurhaeni, and M. Halima, “Isolasi, Identifikasi, dan Karakterisasi Jamur Entomopatogen dari Larva Spodoptera Litura (Fabricius),” Bionatura-Jurnal Ilmu-ilmu Hayati dan Fisik, vol. 12, no. 3, pp. 136–141, 2010.
- Xiao, Z. Zheng, H. Yu, W. Huang, and W. Ji, “Microwave-Assisted Biosynthesis of Nano Silver and its Synergistic Antifungal Activity Against Curvularia lunata,” Front Mater, vol. 10, 2023, doi: 10.3389/fmats.2023.1192609.