Abstract :
In the present study biodegradation of Polycyclic Aromatic Hydrocarbon (PAH) compound Naphthalene by four bacterial cultures Bacillus subtilis PD6, Bacillussp. PD9, Enterobactersp. PD11 and Bacillussp. PD14 has been targeted. Biodegradation of Naphthalene by these four selected bacterial cultures was analysed by HPLC (High Performance Liquid Chromatography) technique. HPLC analysis revealed biodegradation of naphthalene by all the four bacterial cultures within a span of six days. Highest biodegradation 78.1% has been shown by Bacillus subtilis PD6 while other bacterial cultures Bacillus sp. PD9 has shown 77.90%, Enterobacter sp. PD11 showed 74.4% and Bacillus sp. PD14 exhibited 73.5% biodegradation of naphthalene.
Keywords :
Bacterial degradation, Biodegradation, Bioremediation., High Performance Liquid Chromatography, HPLC, Naphthalene biodegradation, PAHs, Polycyclic Aromatic HydrocarbonsReferences :
- Balaram Mohapatra and Prashant S. Phale (2021) Microbial Degradation of Naphthalene and Substituted Naphthalenes: Metabolic Diversity and Genomic Insight for Bioremediation, Frontiers in Bioengineering and Biotechnology. Vol. 9
- Banerjee, D. K., Fedorak P. M., Hashimoto A., Masliyah J. H., Pickard M. A., and Gray M. R (1995) Monitoring the biological treatment of anthracene-contaminated soil in a rotating-drum bioreactor. Appl. Microbiol. Biotechnol43:521–528.
- Bogan B.W., Lahner L.M., Sullivan W.R. and Paterek J.R. (2003) Degradation of straight-chain aliphatic and high-molecular weight polycyclic aromatic hydrocarbons by a strain of Mycobacterium austroafricanum, Journal of Applied Microbiology 94:230–239.
- Bos R. P., Theuws J. L. G., Leijdekkers C.-M. and Henderson P. T. (1984) The presence of the mutagenic polycyclic aromatic hydrocarbons benzo- [a]pyrene and benz[a]anthracene in creosote P1. Mutat. Res. 130:153–158.
- Bossert, I. D., and Bartha R. (1986) Structure biodegradability relationships of polycyclic aromatic hydrocarbons in soil. Bull. Environ. Contam. Toxicol. 37:490–495.
- Boxall, A. B. A., and Maltby L. (1997) The effects of motorway runoff on freshwater ecosystems. 3. Toxicant confirmation. Arch. Environ. Contam. Toxicol. 33:9–16.
- Cerniglia, C. E.1992. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3:351–368.
- Cerniglia, C. E., and Heitkamp M. A. (1989) Microbial degradation of polycyclic aromatic hydrocarbons (PAH) in the aquatic environment, p.41–68. In U. Varanasi (ed.), Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment. CRC Press, Inc., Boca Raton, Fla.
- Chikere C. B., Okpokwasili G. C. and Chikere B. O., (2009) Bacterial diversity in a tropical crude oil-polluted soil undergoing bioremediation. African Journal of Biotechnology 8(11):2535-2540.
- Churchill Sharon A., Harper Jennifer P.,And Churchill Perry F. (1999) Isolation and Characterization of a Mycobacterium Species Capable of Degrading Three- and Four-Ring Aromatic and Aliphatic Hydrocarbons applied and environmental microbiology 65(2):549–552.
- Coates D. John, Woodward Joan, Allen Jon, Philp Paul, and Lovley R. Derek., (1997) Anaerobic Degradation of Polycyclic Aromatic Hydrocarbons and Alkanes in Petroleum-Contaminated Marine Harbor Sediments. Applied and Environmental Microbiology 63(9):3589–3593.
- Deschênes, L., Lafrance P., Villeneuve J.-P., and Samson R. (1996) Adding sodium dodecyl sulfate and Pseudomonas aeruginosaUG2 biosurfactants inhibits polycyclic aromatic hydrocarbon biodegradation in a weathered creosote-contaminated soil. Appl. Microbiol. Biotechnol. 46:638–646.
- Eaton Richard W. (2001) Plasmid-Encoded Phthalate Catabolic Pathway InArthrobacterKeyseri12B. Journal of Bacteriology 183(12):3689–3703.
- Edwards, N. T. (1983) Polycyclic aromatic hydrocarbons (PAHs) in the terrestrial environment—a review. J. Environ. Qual. 12:427–441.
- FantroussiSaïd EL and Agathos Spiro N (2005) Isbioaugmentation a feasible strategy for pollutant removal and site remediaton? Curr Opinion Microbiol 8: 268-275.
- Franco Gianluca, 2009, http://flipper.diff.org/app/items/info/2252
- Gündel, J., Mannschreck C. K., Büttner U., Ewers, and Angerer J. (1996) Urinary levels of 1-hydroxypyrene, 1-, 2-, 3-, and 4-hydroxyphenanthrene in females living in an industrial area of Germany. Arch. Environ. Contam. Toxicol. 31:585–590.
- Hamme Van Jonathan D., Singh Ajay, and Owen P. Ward (2003) Recent Advances in Petroleum Microbiology. Microbiology and Molecular Biology Reviews 67(4):503–549.
- Hatzianestis and Sklivagou E. (2002). Dissolved and Suspended Polycyclic Aromatic Hydrocarbons (pah) in the North Aegean SeaMediterranean Marine Science. 3/1:89-98.
- Hunter Rochelle D., Ekunwe Stephen I. N., Dodor Daniel E., HwangHuey-Minand Ekunwe Lynette (2005) Bacillus subtilis is a Potential Degrader of Pyrene and Benzo[a]pyrene. Int. J. Environ. Res. Public Health 2(2), 267–271.
- Kanaly Robert A. And Harayama Shigeaki, (2000) Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria. Journal of Bacteriology 182(8):2059–2067.
- Keith, L. H., and Telliard W. A. (1979) Priority pollutants I—a perspective view. Environ. Sci. Technol. 13:416–423.
- Koeber, R., Bayona J. M., and Niessner R. (1999) Determination of benzo[a]pyrenediones in air particulate matter with liquid chromatography mass spectrometry. Environ. Sci. Technol. 33:1552–1558.
- KosaricNaim(2001) Biosurfactants and Their Application for Soil Bioremediation, Food Technol. Biotechnol. 39 (4):295–304.
- Laura Dobos, Puia Carmen (2011) The Effect of Bioremediation on the Microbial Consortia of Oil Polluted Soil. ProEnvironment 4:182 – 185.
- Lim L. H., Harrison R. M., and Harrad S. (1999) The contribution of trafficto atmospheric concentrations of polycyclic aromatic hydrocarbons. Environ.Sci. Technol. 33:3538–3542.
- McKenna E. J. (1976) Biodegradation of Polynuclear Aromatic Hydrocarbon Pollutants By Soil And Water Microorganism WRC Research Report No. 113.
- Molina M. Carmen, González Natalia L., Bautista Fernando, Sanz Raquel, Simarro Raquel, Sánchez Irene, Sanz José L. (2009) Isolation and genetic identification of PAH degrading bacteria from a microbial consortium. Biodegradation 20:789–800.
- Paxéus Nicklas (1996) Vehicle Washing As a Source of Organic Pollutants in Municipal Wastewater, Wat. Sci. Tech. 33(6):1-8.
- Phillips, D. H. 1983. Fifty years of benzo[a]pyrene. Nature 303:468–472.
- Potter C. L., Glaser J. A., Chang L. W., Meier J. R., Dosani M. A., and Herrmann R. F. (1999) Degradation of polynuclear aromatic hydrocarbons under bench-scale compost conditions. Environ. Sci. Technol. 33:1717–1725.
- Salihu A., Abdulkadir I. and Almustapha M. N. (2009) An investigation for potential development on biosurfactants. Biotechnology and Molecular Biology Reviews 3(5):111-117.
- SarmaPriyangshuManab, Bhattacharya Dhruva, Krishnan S., and LalBanwari (2004) Degradation of Polycyclic Aromatic Hydrocarbons by a Newly Discovered Enteric Bacterium, Leclerciaadecarboxylata., Applied and Environmental Microbiology 70 (5):3163–3166.
- Sayler Gary S., Hooper Scott W., Layton Alice C. and King J. M. Henry (1990) Catabolic plasmid of environmental and ecological significance, MicrobEcol 19:1-20.
- Shuttleworth, K. L., and Cerniglia C. E. (1995) Environmental aspects of PAH biodegradation. Appl. Biochem. Biotechnol. 54:291–302.
- Singleton David R., Ramirez Liza Guzma´n, and Aitken Michael D., (2009), Characterization of a Polycyclic Aromatic Hydrocarbon Degradation Gene Cluster in a Phenanthrene-Degrading Acidovorax Strain Applied And Environmental Microbiology 75(9):2613–2620.
- Tabatabaee A, Assadi M Mazaheri, Noohi A A, Sajadian V A (2005)Isolation of Biosurfactant Producing Bacteria from Oil Reservoirs,Env Iranian J Health SciEng2(1):6-12.
- Toledo F.L., Calvo C., Rodelas B., González-LópezJ. (2006) Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. Systematic and Applied Microbiology 29 244–252.
- Twiss M. R., Granier L., Lafrance P., and Campbell P. G. C. (1999). Bioaccumulation of 2,29,5,59-tetrachlorobiphenyl and pyrene by picoplankton (Synechococcusleopoliensis, Cyanophyceae): influence of variable humic acid concentrations and pH. Environ. Toxicol. Chem. 18:2063–2069.
- Veľková Veronika, Bubeníková Tatiana, and Výbohová Eva, (2008), Environmental Impact Of Polycyclic Aromatic Hydrocarbons From Wood-Impregnation Division, Proceedings of Ecopole 2(1): 147-151.
- Wagrowski, D. M., and Hites R. A. (1997) Polycyclic aromatic hydrocarbon accumulation in urban, suburban, and rural vegetation. Environ. Sci. Technol. 31:279–282.
- Widada J., Nojiri H., Omori T. (2002) Recent developments in molecular techniques for identification and monitoring of xenobiotic-degrading bacteria and their catabolic genes in bioremediation, ApplMicrobiolBiotechnol (2002) 60:45–59.
- Wilson, S. C., and Jones K. C. (1993) Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): a review. Environ. Pollut. 81:229–249.