Abstract :
The primary means of nutrient uptake for cyanobacteria is oxygen-producing photosynthesis. Their ecological variety is astounding; they occupy a very wide range of lighted ecological niches in terrestrial, marine, and freshwater habitats. Despite this apparent metabolic consistency, they exhibit tremendous phylogenetic diversity. The fact that cyanobacteria have certain physiological and metabolic traits that are exclusively seen in prokaryotes significantly broadens this spectrum. The capacity to fix nitrogen in an aerobic manner under light is a special characteristic. Apart from Gonotheca species, all aerobic, nitrogen-fixing cyanobacteria—a structurally diverse group—produce heterocyst, the highly specialised cells that allow them to fix nitrogen efficiently in a fully aerobic environment by preventing the oxygen-sensitive enzyme nitrogenase from being inactivated quickly in vivo (Hazelton, 1978; Stanier and Cohen-Bazire, 1978; Stewart, Haystead, and Pearson, 1969). The same quantities of cyanobacteria could be isolated from freshwater using a technique using nutrient-saturated glass fibre filters, but the quantity of accompanying heterotrophic bacteria was reduced by 2- to 15-fold. a broad-spectrum antibiotic called imipenem. In comparison to some other Plactam antibiotics, the B-lactam antibiotic that inhibits peptidoglycan biosynthesis, was more effective at lowering the levels of heterotrophic bacterial contaminants associated with newly isolated cyanobacteria to a point that made it easier to grow axenic cyanobacterial cultures.
Keywords :
Blue Green Algae, Cyanobacteria, Photosynthetic Bacteria, Prokaryotic.References :
- Allen, E. A. D., and P. R. Gorham. 1981. Culture of planktoniccyanophytes on agar, p. 185-192. In W. W. Carmichael (ed.), The water environment: algal toxins and health. Plenum Publishing Corp., New York.
- Allen, M. B. 1952. The cultivation of Myxophyceae. Arch.Mikrobiol. 17:34-53.
- Allen, M. M. 1968. Simple conditions for growth of unicellularblue-green algae on plates. J. Phycol. 4:1-4.
- Allen, M. M., and R. Y. Stanier. 1968. Selective isolation ofblue-green algae from water and soil. J. Gen. Microbiol. 51:203-209.
- Allison, F. E., and H. J. Morris. 1930. Nitrogen fixation by blue-green algae. Science 71:221-223.
- Bowyer, J. W., and V. B. D. Skerman. 1968. Production of axenic cultures of soil-borne and endophytic blue-green algae. J. Gen. Microbiol. 54:299-306.
- Bretscher, A. P., and D. Kaiser. 1978. Nutrition of Myxococcus xanthus, a fruiting myxobacterium. J. Bacteriol. 133:763-768.
- Carmichael, W. W., and P. R. Gorham. 1974. An improved method for obtaining axenic clones of planktonic blue-greenalgae. J. Phycol. 10:238-240.
- Castenholz, R. W. 1988. Culturing methods for cyanobacteria.Methods Enzymol. 167:68-93.
- Castenholz, R. W., R. Lewin, R. Rippka, J. B. Waterbury, and B. A. Whitton. 1989. Oxygenic photosynthetic bacteria, p.1710-1806. In J. T. Staley, M. P. Bryant, N. Pfennig, and J. G.Holt (ed.), Bergey’s manual of systematic bacteriology, vol. 3.
- The Williams & Wilkins Co., Baltimore..Ferris, M. J., and C. F. Hirsch. 1989. Abstr. Annu. Meet. Am.Soc. Microbiol. 1989, 1-54, p. 226.
- Fogg, G. E. 1942. Studies on nitrogen fixation by blue-green algae. 1. Nitrogen fixation by Anabaena cylindrica Lemm. J. Exp. Biol. 19:78-87.
- Gerloff, G. C., G. P. Fitzgerald, and F. Skoog. 1950. The- isolation, purification, and culture of blue-green algae. Am. J.Bot. 37:216-218.
- Heaney, S. I., and G. H. M. Jaworski. 1977. A simple separation technique for purifying micro-algae. Br. Phycol. J. 12:171-174.
- Kraus, M. P. 1966. Preparation of pure blue-green algae. Nature (London) 211:310.
- Krieg, N. R., and P. Gerhardt. 1981. Solid culture, p. 143-150.In P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester,
- A. Wood, N. R. Krieg, and G. B. Phillips (ed.), Manual of methods for general bacteriology. American Society for Microbiology,Washington, D.C.
- Kropp, H., L. Gerkens, J. G. Sundelof, and F. M. Kahan. 1985. Antibacterial activity of imipenem: the first thienamycin antibiotic.Rev. Infect. Dis. 7:389-410.
- Kropp, H., J. G. Sundelof, J. S. Kahan, F. M. Kahan, and J.Birnbaum. 1980. MK0787 (N-formimidoyl thienamycin): evaluation of in vitro and in vivo activities. Antimicrob. Agents Chemother. 17:993-1000.
- McDaniel, H. R., J. B. Middlebrook, and R. 0. Bowman. 1962.Isolation of pure cultures of algae from contaminated cultures. Appl. Microbiol. 10:223.
- Meffert, V. M.-E., and T.-P. Chang. 1978. The isolation Fplanktonic blue green algae (Oscillatoria-species). Arch.Hydrobiology 82:231-239.
- Parker, D. L. 1982. Improved procedures for the cloning andpurification of Microcystis cultures (cyanophyta). J. Phycol. Pereira, I., Ortega, R, Barrientos, L., Moya, M., Reyes, G. and Kramm, V., Development of a Biofertilizer Based on Filamentous Nitrogen-Fixing Cyanobacteria for Rice Crops in Chile, J. Appl. Phycol., Vol.21, pp.135-144, 2009.
- Karthikeyan, N., Prasanna, R., Nain, L. and Kaushik, B.D., Evaluating the Potential of Plant Growth Promoting Cyanobacteria as Inoculants for Wheat,.23-30, 2007.
- Osman, M.E.H., El-Sheekh, M.M., El-Naggar, A.H., and European Journal of Soil Biology, Vol.43, pp Gheda, S.F., Effect of Two Species of Cyanobacteria as Biofertilizers on Some Metabolic Activities, Growth, and Yield of Pea Plant, Biol. Fertil. Soils, Vol.46, pp.861-875, 2010.
- Maqubela, M.P., Mnkeni, P.N.S., Malam Issa,O., Pardo, M.T. and D’Acqui, L.P., Nostoc Cyanobacterial Inoculation in South African Agricultural Soils Enhances Soil Structure, Fertility, and Maize Growth, Plant Soil, Vol.315, pp.79-92, 2009.
- Maqubela, M.P., Muchaonyerwa, P and Mnkeni, P.N.S., Inoculation Effects of Two South African Cyanobacteria Strains on Aggregate Stability of a Silt Loam Soil, African Journal of Biotechnology, Vol.11, No.47, pp.10726-10735, 2012.
- Prasanna, R., Joshi, M., Rana, A., and Nain, L., Modulation of IAA Production in Cyanobacteria by Tryptophan and Light, Polish Journal of Microbiology, Vol.59, No.2, pp. 99-105, 2010.
- Kumar, C.P., Yashoda, P., Dinesh, P. and Shashwat, N., Study of Soil Cyanobacteria to Evaluate Metabolite Production during Various Incubations in their Culture Filtrate, Scholars Academic journal of Biosciences, Vol.1, No.5, pp.154-158, 2013