Abstract :

Food preservatives are unswervingly being used for prolonging the shelf life of food, often to increase the aroma, taste and quality by food packaging industry. These food preservatives are pivotal in protecting food from degradation and deterioration by micro-organisms. Two of the most commonly used food preservatives, viz. sodium nitrite (NaNO₂) and Monosodium glutamate (MSG) have been evaluated using root tips of Allium cepa. Cytological studies in plants offer a first-tier bioassay that are sensitive and reliable. Allium cepa is used as an experimental model as it shows chromosomal aberrations and other morphological abnormalities as that of the mammalian systems. Dose and time dependent evaluation of the mitotic damage was done on the root tips of onion (Allium cepa). Mitotic Index (MI) was calculated based on the cytological observation post-treatment with their respected controls. Chromosomal abnormalities like chromosomal bridge and laggard, multipolarity and stickiness in the chromosome was observed in the treated sample.

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Keywords :

Allium cepa, Mitotic index (MI), Monosodium glutamate (MSG), Sodium nitrite (NaNO2).

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References :

1. Adeyemo OA, Farinmade AE. Genotoxic and cytotoxic effects of food flavor enhancer, monosodium glutamate (MSG) using Allium cepa assay. African Journal of Biotechnology. 2013; 12(13):1459-66.
2. Fuke S, Shimizu T. Sensory and preference aspects of umami. Trends Food Sci. Technol. 1993; 4:246-251.
3. Yamaguchi S, Ninomiya K. Umami and food palatability. J. Nutr. 2000; 130 (4): 921S926S.
4. Filer LJ, Stegink LD. Report of the proceedings of the glutamate workshop. Crit. Rev. Food Sci. Nutr. 1994; 34(2):159-174.
5. Fernstrom JD, Garattini S. International symposium on glutamate. J. Nutr. 2000;130 (Suppl 4): 891S–1079S.
6. Freeman M. Reconsidering the effects of monosodium glutamate. A literature review. J. Am. Acad. Nurse Pract. 2006; 18(10): 482- 486.
7. Yang WH, Drouin MA, Herbert M, Mao Y, Karsh J. The monosodium glutamate symptom complex: assessment in a double blind placebo-controlled, randomised study. J. Allergy Clin. Immunol. 1997; 99:757-762.
8. El-Wakf AM, Hassan HA, Elsaid FG, El-Said A. Hypothyroidism in Male Rats of Different Ages Exposed to Nitrate Polluted Drinking Water. Research Journal of Medicine and Medical Sciences. 2009; 4(2): 160-164.
9. Chauhan LKS, Sundararaman V. Effects of substituted ureas on plant cells I. Cytological effects of isopruturon on the root meristem cells of Allium cepa, Cytologia (1990); 55:91–98.
10. Fiskesjӧ G. The Allium test-an alternative in environmental studies: the relative toxicity of metal ions. Mutation Res.1988; 197(2): 243-260.
11. Jones RN, Rickards GK. Practical genetics. Open University press, Philadelphia. 1991.
12. Grant WF. Chromosomal aberrations in plants as a monitoring system. Environ. Hea. Persp. 1978; 27: 37-43.
13. Auti S, Pagare R, Ahire D, Sawale V. Cytogenetical studies on the effect of omnacortil on root tip cells of Allium cepa L. Journal of Cell and Tissue Research. 2010; 10(3): 23312335.
14. Kumar LP, Panneerselvam N. Cytogenetic studies of food preservative in Allium cepa root meristem cells. Med. Biol. 2007; 14(2):60- 63.
15. Türkoglu S. Genotoxicity of five food preservatives tested on root tips of Allium cepa L. Mutat. Res. 2007; 626: 4–14.
16. Nagwa RAH, Magda AME, Atef AAH, Elham AAAH. Relative Mutagenecity of Some Food Preservatives on Plant Cells. Aust. J. Basic Appl. Sci. 2011; 5(12):2817-2826
17. Kumar G, Srivastava N. Genotoxic effects of two commonly used food additives of boric acid and sunset yellow in root meristems of Trigonella foenum-graecum. Iran. J. Environ. Health. Sci. Eng. 2011; 8(4):361-366.
18. Grant WF. Higher plant assays for the detection of chromosomal aberrations and gene mutations-A brief historical; background on their use for screening and monitoring environmental chemicals. Mutat. Res. 1999; 426:107-112.
19. Odeigah PGC, Makinwa J, Lawal B, Oyeniyi R. Genotoxicity screening of leachates from solid industrial waste evaluated Allium test. ATLA 1997; 25:311-321.
20. Fiskesjö G. The Allium test as a standard in environmental monitoring. Hereditas. 1985; 102: 99–112.
21. Bakare AA, Adeyemo AR. The potential mutagenic and cytotoxic effects of leachate from domestic wastes and Aba-Eku landfill, Nigeria on Allium cepa. Nature Environ. Pollut. Technol. 2004; 3(4):455- 462.