Abstract :

This research was conducted to determine the effect of fermentation time on lactic acid bacteria (LAB), pH, total titrated acid (TTA) and organoleptic (taste, flavor, color and texture) of cowpea soft cheese (Vigna unguiculata L.Walp) with starter culture Lactobacillus plantarum B1765. L.plantarum B1765 had been studied has a probiotic characteristics.   The fermentation time was 0, 6, 18 and 24 hours. The methods were total plate count (TPC) to measure the number of LAB, pH meter to measure pH, acid-base titration to measure TTA, and hedonic test for organoleptic. The results showed that the fermentation time affected the number of LAB, pH, TTA and organoleptic. The number of LAB increased by 3 log cycles from 8.7 x 10⁶ CFU/mL to 5,3 x 10⁹ CFU/mL. The pH value decreased from 6.53 to 3.69. The TTA value increased from 0.45% to 1.79%. The highest level of preference for taste, texture, color and flavor was at 18 hours of fermentation with a value of 3.17; 2.77; 2.70; 2.97 (likes). Cowpea soft cheese with starter L.plantarum B1765  could be improve potentially  as vegetable cheese and  probiotic agent.

 

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Keywords :

Cowpea, L. plantarum B1765, Probiotic, Product Quality, Soft Cheese.

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References :

1. Syamsu and K. Elsahida, “Pembuatan Keju Nabati Dari Kedelai Menggunakan Bakteri Asam Laktat Yang Diisolasi Dari Dadih,” J. Teknol. Ind. Pertan., vol. 28, no. 2, pp. 154–161, 2018, doi: 10.24961/j.tek.ind.pert.2018.28.2.154.
2. Ardelia, T. Pantjajani, and F. Irawati, “Pembuatan Keju Lunak Kacang Merah (Phaseolus Vulgaris L.) dengan Proses Fermentasi Menggunakan Lactobacillus Acidophillus,” vol. 9, no. 1, 2020.
3. Nurgrahadi, N. N. Puspawati, and I. M. Sugitha, “Pengaruh Perlakuan 3 Jenis Bakteri Asam Laktat Dan Kombinasinya Terhadap Karakteristik Keju Kedelai,” Ilmu dan Teknol. Pangan, vol. 9, no. 4, p. 412, 2020,

doi: 10.24843/itepa.2020.v09.i04.p06.

4. Pravitasari, D. Hariyadi, and M. Mulyanita, “Daya Terima Sari Kacang Hijau (Phaseolus Radiatus L) Sebagai Bahan Alternatif Pembuatan Keju,” Pontianak Nutr. J., vol. 3, no. 2, p. 34, 2020, doi: 10.30602/pnj.v3i2.696.
5. G. A. Sujana, N. S. Antara, and I. B. W. Gunam, “Skrining Isolat Bakteri Asam Laktat Penghasil Bakteriosin yang Diisolasi dari Asinan Rebung Bambu Tabah dan Ketahanannya terhadap Panas,” J. Rekayasa Dan Manaj. Agroindustri, vol. 8, no. 4, p. 615, 2020, doi: 10.24843/jrma.2020.v08.i04.p15.
6. Sharma, D. Sharma, and A. Amin, “Development of a functional fermented peanut-based cheese analog using probiotic bacteria,” Biotechnologia, vol. 99, no. 4, pp. 435–441, 2018, doi: 10.5114/bta.2018.79973.
7. Fadillah, H. Purnamawati, and Supijatno, “Produksi Kacang Tunggak (Vigna unguiculata [L.] Walp) dengan Input Pupuk Rendah,” J. Agron. Indones. (Indonesian J. Agron., vol. 48, no. 1, pp. 44–51, 2020, doi: 10.24831/jai.v48i1.27597.
8. AhliGiziID, “Nilai kandungan gizi Kacang tolo / tunggak,” com, 2018.

https://nilaigizi.com/gizi/detailproduk/277/kacang-tolo-tunggak (accessed Jul. 16, 2021).

9. K. K. Y. Sujadmiko and P. R. Wikandari, “Resistensi Antibiotik Amoksilin Pada Strain Lactobacillus plantarum B1765 Sebagai Kandidat Kultur Probiotik,” Unesa J. Chem., vol. 6, no. 1, May 2017.
10. Y. Adriane and P. R. Wikandari, “Pengaruh Konsentrasi Lactobacillus plantarum B1765 terhadap Mutu Produk Keju Analog Kacang Kecipir (Psophocarpus tetragonolobus),” pp. 34–45, 2021.
11. Setiawati, H. Rizqiati, and S. Susanti, “Analisis Rendemen, Kadar Alkohol, Nilai pH dan Total BAL pada Kefir Whey Susu Kambing dengan Lama Fermentasi yang Berbeda,” J. Teknol. Pangan, vol. 3, no. 1, pp. 142–146, 2019.
12. ISO 19344, “Milk and milk products — Starter cultures, probiotics and fermented products — Quantification of lactic acid bacteria by flow cytometry,” 2015. https://www.iso.org/obp/ui/#iso:std:iso:19344:ed-1:v1:en (accessed Apr. 08, 2022).
13. Badan Standarisasi Nasional, “SNI 2980 : 2018-Keju Olahan,” vol. ICS 67.100, 2018.
14. Suhardi, Kimia dan Teknologi Protein. Yogyakarta: PAU Pangan dan Gizi UGM, 1991.
15. R. Agustin, “Karakterisasi Konsentrat Protein Kacang Tunggak (Vigna Unguiculata L. Walp) Dari Berbagai Fraksi Protein Berdasarkan Kelarutan – Brawijaya Knowledge Garden,” Univ. Brawijaya, 2014.
16. Edam, “Pengaruh Kombinasi Konsentrsi NaCl dan Lama Fermentasi Terhadap Produksi Asam Laktat dari Kubis ( Brassica oleracea ),” J. Penelit. Teknol. Ind., vol. 10, no. 1, pp. 17–24, 2018.
17. D. Belitz, W. Grosch, and P. Schieberle, Food Chemistry, 4th revise. Berlin: Springer-verlay, 2009.