Abstract :
There are several factors that influence the production of biohydrogen by dark fermentation including inoculum seeds, type and concentration of substrate, pH, temperature, presence of micronutrient and reactor configuration. Previous research has proven that the concentration of substrate and the presence of micronutrient will influence the yield and productivity of biohydrogen production. However, improvement of yield and productivity of the process can only be achieved once the system is under the optimum amount of substrate and micronutrient. Therefore, the best way to determine the effect of substrate concentration and presence of micronutrient is through kinetic study that was done using Monod model along with Andrews model. Besides that, the substrate inhibition effect also will be evaluated to determine the maximum substrate that needs to be supplied for maximum hydrogen production, and thus supplied the information for economic feasibility for fermentation process. In the meantime, the inhibition effect of adding the iron nanoparticles also had been evaluated in order to understand the interaction effect between iron nanoparticles and bacteria in term of catabolism reaction. It was found that increasing the substrate concentration more than 10 g/l will cause the inhibition to the system, in which it will slow down the reaction process and reduced the production of hydrogen. While the presence of iron NPs more than its optimum value (200 mg/l) will inhibit the bacterial growth and hence, affect the hydrogen production. For both cases, when the inhibition occurred at the respective concentration, it was found that the metabolic pathway was shifted to produce more hydrogen-consuming metabolite such as propionate acid, and thus, dropped the hydrogen production.
Keywords :
Anaerobic Fermentation, Biogas, Inhibition, Kinetic StudyReferences :
1. D. B. Levin, L. Pitt, and M. Love, “Biohydrogen production : prospects and limitations to practical application,” vol. 29, pp. 173–185, 2004, doi: 10.1016/S0360-3199(03)00094-6.
2. S. Van Ginkel, S. Sung, and J. Lay, “Biohydrogen Production as a Function of pH and Substrate Concentration,” vol. 35, no. 24, pp. 4726–4730, 2001.
3. A. A. I. Luthfi, J. M. Jahim, S. Harun, J. P. Tan, S. F. A. Manaf, and S. S. M. Shah, “Kinetics of the bioproduction of succinic acid by actinobacillus succinogenes from oil palm lignocellulosic hydrolysate in a bioreactor,” BioResources, vol. 13, no. 4, pp. 8279–8294, 2018, doi: 10.15376/biores.13.4.8279-8294.
4. A. L. Koch, “The Monod Model and Its Alternatives,” Math. Model. Microb. Ecol., no. 2, pp. 62–93, 1998,
doi: 10.1007/978-1-46154078-6_4.
5. J. F. Andrews, “A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates,” Biotechnol. Bioeng., vol. 10, no. 6, pp. 707–723, 1968, doi: 10.1002/bit.260100602.
6. V. Niel, E. W. J. Van Niel, P. A. M. Claassen, and A. J. M. Stams, “Substrate and Product Inhibition of Hydrogen Production by the Extreme Thermophile, Caldicellulosiruptor saccharolyticus,” 2003, doi: 10.1002/bit.10463.
7. S. N. Malik, V. Pugalenthi, A. N. Vaidya, P. C. Ghosh, and S. N. Mudliar, “Kinetics of nano-catalysed dark fermentative hydrogen production from distillery wastewater,” Energy Procedia, vol. 54, pp. 417–430, 2014,
doi: 10.1016/j.egypro.2014.07.284.
8. K. Reddy et al., “Biohydrogen production from sugarcane bagasse hydrolysate: effects of pH, S/X, Fe2+, and magnetite nanoparticles,” Environ. Sci. Pollut. Res., vol. 24, no. 9, pp. 8790–8804, 2017, doi: 10.1007/s11356-017-8560-1.
9. I. Ntaikou, H. N. Gavala, M. Kornaros, and G. Lyberatos, “Hydrogen production from sugars and sweet sorghum biomass using Ruminococcus albus,” vol. 33, pp. 1153–1163, 2008, doi: 10.1016/j.ijhydene.2007.10.053.
10. K. Han and O. Levenspiel, “Extended Monod Kinetics for Substrate Product, and Cell Inhibition,” in Biotechnology and Bioengineering, 32nd ed., Oregon: John Wiley & Sons, Inc., 1987, pp. 430–437.
11. J. Wang and W. Wan, “Factors influencing fermentative hydrogen production: A review,” Int. J. Hydrogen Energy, vol. 34, no. 2, pp. 799–811, 2009, doi: 10.1016/j.ijhydene.2008.11.015.
12. N. S. Engliman, P. M. Abdul, and S. Wu, “Influence of iron (II) oxide nanoparticle on biohydrogen production in thermophilic mixed fermentation,” Int. J. o f h ydrogen e nergy xxx 1e12, no. Ii, pp. 1–12, 2017.
13. S. Wu, C. Lin, J. Chang, and J. Chang, “Biohydrogen production with anaerobic sludge immobilized by ethylene-vinyl acetate copolymer,” vol. 30, pp. 1375–1381, 2005, doi: 10.1016/j.ijhydene.2004.09.011.
14. Y. Akutsu, Y. Li, H. Harada, and H. Yu, “Effects of temperature and substrate concentration on biological hydrogen production from starch,” Int. J. Hydrogen Energy, vol. 34, no. 6, pp. 2558–2566, 2009,
doi: 10.1016/j.ijhydene.2009.01.048.
15. K. Nath, M. Muthukumar, A. Kumar, and D. Das, “Kinetics of two-stage fermentation process for the production of hydrogen,” Int. J. Hydrogen Energy, vol. 33, no. 4, pp. 1195–1203, 2008, doi: 10.1016/j.ijhydene.2007.12.011.
16. J. Wang and W. Wan, “Kinetic models for fermentative hydrogen production: A review,” Int. J. Hydrogen Energy, vol. 34, no. 8, pp. 3313–3323, 2009, doi: 10.1016/j.ijhydene.2009.02.031.
17. Y. C. Lo, W. M. Chen, C. H. Hung, S. Der Chen, and J. S. Chang, “Dark H2 fermentation from sucrose and xylose using H2-producing indigenous bacteria: Feasibility and kinetic studies,” Water Res., vol. 42, no. 4–5, pp. 827–842, 2008, doi:
18. 10.1016/j.watres.2007.08.023.
19. A. Gadhe, S. S. Sonawane, and M. N. Varma, “Kinetic analysis of biohydrogen production from complex dairy wastewater under optimized condition,” Int. J. Hydrogen Energy, vol. 39, no. 3, pp. 1306–1314, 2014,
doi: 10.1016/j.ijhydene.2013.11.022.
20. Y. Mu, G. Wang, and H. Q. Yu, “Kinetic modeling of batch hydrogen production process by mixed anaerobic cultures,” Bioresour. Technol., vol. 97, no. 11, pp. 1302–1307, 2006, doi: 10.1016/j.biortech.2005.05.014.
21. P. Harrison, J. Parslow, and H. Conway, “Determination of nutrient uptake kinetic parameters: a comparison of methods,” Mar. Ecol. Prog. Ser., vol. 52, no. 3, pp. 301–312, 1989, doi: 10.3354/meps052301.
22. J. D. Owens and J. D. Legan, “Determination of the Monod substrate saturation constant for microbial growth,” vol. 46, pp. 419–432, 1987.
23. S. Ozmihci and F. Kargi, “Kinetics of batch ethanol fermentation of cheese-whey powder (CWP) solution as function of substrate and yeast concentrations,” Bioresour. Technol., vol. 98, no. 16, pp. 2978–2984, 2007,
doi: 10.1016/j.biortech.2006.10.005.
24. J. b. s. Haldane, “Enzymes,” no. 2, 1930.
25. D. Frascari et al., “A kinetic study of biohydrogen production from glucose, molasses and cheese whey by suspended and attached cells of Thermotoga neapolitana,” Bioresour. Technol., vol. 147, no. February 2018, pp. 553–561, 2013, doi: 10.1016/j.biortech.2013.08.047.
26. H. Han, M. Cui, L. Wei, H. Yang, and J. Shen, “Enhancement effect of hematite nanoparticles on fermentative hydrogen production,” Bioresour. Technol., vol. 102, no. 17, pp. 7903–7909, 2011, doi: 10.1016/j.biortech.2011.05.089.
27. J. M. Berg, J. L. 1948-2019. Tymoczko, L. Stryer, and N. C. for B. I. (U. S. . T. A.-T. T. -, “Biochemistry.” W.H. Freeman ;, New York, 2002, [Online]. Available: http://catalog.hathitrust.org/api/volumes/oclc/48055706.html.
