Abstract :

An experiment was conducted for the production of protein, bio-fuel and bio-electricity from the culture system of Spirulina platensis (Gomont) in supernatant of three different amount of digested rotten tomato (Solanum lycopersicum), and Kosaric Medium (KM) as control. Three different concentrations such as 25, 50 and 75% rotten tomato were allowed to digest under aeration. After 17 days, the colorless supernatant was screened and taken in 1.0 L conical flask with three replications. Then, Spirulina platensis was inoculated to grow in these three media (treatments) with the addition of 9.0 g/L NaHCO₃ and micronutrients, and also in KM as control for a period of 14 days. The cell weight, optical density, chlorophyll a and total biomass  of spirulina was attained to the  maximum values when grew in KM on the 10th day of culture followed by supernatant of 50% digested rotten tomato (DRT) than in  25 and 75% DRT culture. The chemical properties of the culture media such as pH, salinity, dissolved bio-oxygen, electric conductivity and bio-electricity were increased from first day up to 12th day of experiment. Total biomass of spirulina grown in these media had highly significant (P < 0.01) correlation with cell weight (r = 0.825) and chlorophyll a (r = 0.866) of spirulina. The results showed that the growth performances of S. platensis grown in supernatant of 50% DRT was significantly (P < 0.01) higher than that of spirulina grown in supernatant of 25 and 75% DRT. The percentage of crude protein (55.10 ± 0.45 to 59.90 ± 0.33%) of spirulina grown in supernatant of DRT was little bit higher than that of spirulina cultured in KM (58.40 ± 0.38%). But bio-fuel in terms of crude lipids (16.50 ± 0.31%) of spirulina cultured in supernatant of 50% DRT was almost two and half times higher than that of spirulina grown in KM (crude lipids, 6.30 ± 0.21%). Bio-electricity (300 ±10.20 mV) produced in culture of spirulina in supernatant of 50% DRT was higher than  that recorded in KM (240 ±10.20 mV) followed by 75% DRT and other media. Bio-electricity had directly and strongly significant (p < 0.001) correlation with pH (r = 0.812), dissolved bio-oxygen (r = 0.832), salinity (r = 0.788) and electric conductivity (r = 0.856). Therefore, this procedure will produce huge amount of electricity in the world and will make a revolution in this field of bio-electricity production. Whole world will be benefited from the output (results) of this experiment.

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Keywords :

bio-electricity, bio-fuel, protein, rotten tomato, Spirulina, supernatant

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References :

1. Phang, S. M., Miah, M S., Chu, W. L., and Hashim, A. 2000 Spirulina culture in digested sago starch factory waste water. J. Appl. Phycol. 12, 395-400.
2. Raji, A. A., Alaba, P. A., Yusuf, H., Bakar, N. H. A., Taufek, N. M.,  Muin, H., Alias, Z., Milow, P., and Razak, S. A.  2018 Fishmeal replacement with Spirulina platensis and Chlorella vulgaris in African catfish (Clarias gariepinus) diet: Effect on antioxidant enzyme activities and haematological parameters. Res. Vet. Sci., 119, 67-75.

3. Sarr, S.M., Fall, J., Thiam, A., and Barry, R.O. 2019 Growth and survival of red tilapia (Oreochromis aureus x Oreochromis mossambicus) fry fed on corn and soy meal, peanut meal and fishmeal enriched with spirulina (Spirulina platensis). Int. J. Agric. Policy & Res. 7(1), 1-9.
4. Habib, M. A. B., Parvin, M., T., Huntington, C., and Hasan, M. R. 2008 Global Review on Culture, Production and Use of Spirulina as Food for Humans and Feed for Domestic Animals and Fish. TC Huntington (Editor), Report Number GF FIRID. RA2IP02000600. Food and Agriculture Organization (FAO) of United Nations, Rome, Italy.
5. Muys, M., Sui, Y., Schwaiger, B., Lesueur, C., Vandenheuvel, D., Vermier, P., and Vlaeminck, S.E. 2019 High variability in nutritional value and safety of commercially available Chlorella and Spirulina biomass indicates the need for smart production strategies. Biores. Tech. 275,247-257.
6. Louhasakul, Y., Cheirsilp, B., Maneerat, S., Prasertsan, P. 2018 Direct transesterification of oleaginous yeast lipids into biodiesel: Development of vigorously stirred tank reactor and process optimization. Biochem. Eng. J. 137,232-238.

7. Harris, J., Viner, K., Champagne, P., and Jessop, F. 2018 Advances in microalgal lipid extraction for biofuel production: A review. Bioprod. Bioref, 12,1118–1135.
8. Soydemir, G., Keris-Sen, U. D., Sen, U., and Gurol, M. D. 2015 Biodiesel production of mixed microalgal culture grown in domestic wastewater. Bioprocess Biosystem Eng. 39: 45-51.
9. Idris, S. A., Esat, F. N.,  Rahim, A. A. A.,  Zahin, W.A.,  Ruzlee, R.W., and. Razali, W. M. Z. 2016 Electricity generation from the mud by using microbial fuel cell. MATEc Web of Conference, 69 02001. DoI: 10:1051,matEcconf/20166902001.
10. Hernández-Fernández, F.J., de los Ríos, A. P., Salar-García, M. J., Ortiz-Martínez,   V.M., Lozano-Blanco, L. J., Godínez, C. and Quesada-Medina, J. 2015 Recent progress and perspectives in microbial fuel cells for bioenergy generation and wastewater treatment. Fuel Proc. Tech. 138, 284–297.
11. Liu, W., Cheng, S., Sun, D., Huang, H., Chen, J., and Cen, K. 2015 Inhibition of microbial growth on air cathodes of single chamber microbial fuel cells by incorporating enrofloxacin into the catalyst layer. Biosensors & Bioelectronics 72, 44–50.
12. Thong, C.H., Phang, S. M.,  Ng, F.L., Periasamy, V., Ling, T. C.,  Yunus, K., and Fisher, A. C.  2019 Effect of different irradiance levels on bioelectricity generation from algal biophotovoltaic (BPV) devices. Energy Sci. Eng. Doi: 10.1002/ ese3. 414.
13. Subhashini J, Mahipal, S. V. K., Reddy, M. C., Reddy, M. M., Rachamallu, A., and Reddanna, P. 2004 Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. Biochem. Pharma. 68, 453-462.
14. Alvarenga, R. R., Rodrigues, P.B., de Souza. Cantarelli, V., Zangeronimo, M.G., de Silva Jứnior, J.W., de Silva, L. R., dos Santos, L. M., and Pereira. L.J. 2011 Energy values and chemical composition of spirulina (Spirulina platensis) evaluated with broilers. Revista Brasileira de Zootecnia. 40(5), 992-996.

15. Zahroojian, N., Moravej, H., and Shivazad, M. 2013 Effect of dietary marine algae (Spirulina platensis) and production performance of laying hens. J. Agric. Sci. Tech. 15, 1353-1360.
16. Rosas, V. T., Manserrat, J. M., Bassonart, M., Magnone, L., Romano, L. A.,   Tesser, M. B. 2019 Comparison of ß-carotene and spirulina (Spirulina platensis) in mullet (Mugil liza) diets and effects on antioxidant performance and fillet colouration. J. Appl.  Phycol. Doi:org/101007/s10811-019-01773-1.
17. Syaifudin, N., Nurkholis, R., Handika, and Setyobudi, R. H. 2018 Formulating interest subsidy program to support development of electricity generation from palm oil mill effluent (POME) biomass: an Indonesian case study. MATEC Web Conferences, 164, pp. 1-9.
18. Lu, J., and Takeuchi, T. 2004 Spawning and egg quality of tilapia Oreochromis niloticus fed solely on raw Spirulina throughout three generation. Aquaculture 23(4), 625-640.
19. Habib, M. A. B., and Kohinoor, A. H. M. 2018 Culture and production of house fly larvae and spirulina using poultry waste and their use as food for catfish post-larvae. Report on Advanced Research. Grants for Advanced Research in Education, Secondary and Higher Education Division, Ministry of Education, Government of the Peoples’ Republic of Bangladesh, Vol. 2, Chapter 2, pp. 66-70.
20. Clesceri, L. S., Greenberg, A. E., and Trussell, R. R.  1989, Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association and Water Pollution Control Federation. 17th Edn., 1015 Washington D.C.
21. Horwitz, W. (ed.). 1984 Official methods of Analysis of the Association of Official Analytical chemists. 14th Edn., Association of Official Analytical Chemists, washington D.C.
22. Bold, H. C., and Wynne, M. J. 1978 Introduction to the Algae, Structure and Reproduction. 2nd edition, Prentice-Hall, Inc., Englewood Cliffs, New Jersey.
23. Yamaguchi, T. 1992 Plankton Algae in Taiwan (Formosa). Uchida Rokakuho, Tokyo.
24. Phang, S.M., and Chu, W.L. 1999 University of Malaya Algae Culture Collection (UMACC). Catalogue of Strain. Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia.
25. Zar, J. H. 1984 Biostatistics. Prentice-Hall, Inc., Englewood Cliffs, New Jersey.
26. da Costa, C., and Hadiyanto, H. 2018 Boielectricity production from microalgae-microbial fuel cell technology (MMFC). MATEC Web of Conferences 156, 01017 (2018) https:// doi.org/10.1051/matecconf/201815601017.
27. Lai, M. F.,  Lin, J. H.,  Lou, C. W.,  and  Lin, C. W.  2016  Electricity generation with the novel 3D electrode from swim wastewater in a dual-chamber microbial fuel cell. MATEC Web of Conferences, 01007. Doi: 10.1051/matecconf/2016667 701007
28. Islam, M.A., Rahman, M., Yusuf, A., Cheng, C.K., Wai. W.C.  2016 Performance of Klebsiella oxytoca to generate electricity from POME in microbial fuel cell. MATEC Web of Conference, 38, 03004. Doi: 10.1051/matecconf/2016 3803004.