Abstract :
The aim of this research was to evaluate the effect of addition red beetroot powder (Beta vulgaris L. Var. Rubra L) as a feed additive in feed on production performance, egg yolk cholesterol and blood profile of laying hens. The first stage of research included testing the inhibitory power of Escherichia coli, Salmonella sp, and Lactic Acid Bacteria (LAB). The materials used in the bacterial inhibition test were red beetroot pulp powder, red beetroot peel powder and red beetroot pulp and peel powder. The method used was in vitro research and the data was analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. The second stage of research is the field application of the results of the first stage research in vivo. The material used was 120 laying hens of the isa brown strain aged 30 weeks. The method used was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 6 replications, each using 5 chickens. Data were analyzed statistically using the ANOVA test. If there were differences between each treatment, it was continued with Duncan’s Multiple Range Test. Variables observed in the second stage of research included production performance, egg yolk cholesterol and blood profile of laying hens. The results of the first stage of research showed that the use of red beetroot plant powder had a very significant effect (T<0.01) on the diameter of the inhibition zone for Escherichia coli, Salmonella sp and Lactic Acid Bacteria (LAB). Red Beetroot Pulp and Peel Powder (RBPPP) has the best antibacterial power so it was applied in the second stage of research. The results of the second stage of research showed that the addition of RBPPP had a significant effect (T<0.05) on feed consumption, had a very real effect (T<0.01) on egg weight, HDP, egg mass, feed conversion, IOFC, hemoglobin., erythrocytes and hematocrit. However, there was no significant effect (T>0.05) on egg yolk cholesterol and leukocytes. The addition of RBPPP at the 1% level has the potential to reduce feed consumption and feed convertion ratio, increase egg weight, HDP and egg mass, even though the IOFC value is lower than the control treatment or without RBPPP. The addition of RBPPP at a level of 1.5 % has the potential to reduce egg yolk cholesterol levels compared to other treatments. The addition of RBPPP up to a level of 1 % is able to increase and maintain hemoglobin and hematocrit levels in normal conditions. Apart from that, the addition of RBPPP at levels of 0.5 % and 1.5 % is able to increase and maintain erythrocyte and leukocyte levels in normal conditions.
Keywords :
Blood profile, egg yolk cholesterol, feed additive, laying hens, production performance, Red beetroot pulp and peel powderReferences :
1. Agustina, L and S. Purwanti. 2012. Poultry Nutrition Science. Solo: House of Knowledge.
2. Ali, AS, Ismoyowati and D. Indrasanti. 2013. Number of erythrocytes, hemoglobin and hematocrit levels in various types of local ducks regarding the addition of probiotics in the diet. Animal Husbandry Scientific Journal . 1(3): 1001-1013.
3. Al-waeli, SKJ, MHA Alasadi and RJ Abbas. 2021. Effect of supplementing beetroot ( Beta Vulgaris Rubra ) powder and its aqueous extract on productive performance of growing geese. IOP Conference Series: Earth and Environmental Science . 923:012028.
4. Amila., M. Siti., S. Henny., KM Jon and IG Vierto. 2021. Get to know beautiful beets and their benefits . Malang: Media Expert.
5. Ardiansyah, F., S. Tantalo and K. Nova. 2013. Comparison of the performance of two strains of medium type roosters fed commercial broiler rations. Integrated Animal Husbandry Scientific Journal . 1(1): 158-163.
6. Arfah, NM 2015. The Effect of Giving Turmeric Flour in the Diet on the Number of Erythrocytes, Hemoglobin, PVC and Leukocytes in Broiler Chickens. Thesis . Faculty of Veterinary Medicine, Hasanuddin University. Makassar.
7. Central Bureau of Statistics. 2022. Egg production from laying hens in Indonesia (Tons), 2000-2022. Jakarta: Central Statistics Agency.
8. Bozkurt, M., K. Kucukyilmaz., M. Pamukcu., M. Cabuk., A. Alcicek and AU Catli. 2012. Long-term effect of dietary supplementation with an essential oil mixture on the growth and laying performance of two layer strains. Italian Journal of Animal Science. 11(5): 23-28.
9. Canadanovic-Brunet, J. M., S. S. Savatovic., G. S. Cetkovic., J. J. Vulic., S. M. Djilas and S. L. Markov. 2011. Antioksidant and antimicrobial activities of beet root pomace etracts. Czech Journal of Food Sciences. 29: 575-585.
10. Clifford, T., G. Howatson., D. J. West and E. J. Stevenson. 2015. The potential benefits of red beetroot supplementation in healthy and disease. Nutrients. 7(28): 1-22.
11. Eumkeb, G and S. Chukrathok. 2013. Synergistic activity and mechanism of action of ceftazidime and apigenin combination against ceftazidime-resistant Enterobacter cloacae. Phytomedicine. 20(3-4): 262-269.
12. Haryuni., E. Widodo and E. Sudjarwo. 2017. Effect of adding betel leaf juice ( Piper bettle linn ) as a feed additive on the performance of laying hens. Research & Conceptual Journal . 2(4): 429-433.
13. Hasjidla, NF, I. Cholissodin and AW Widodo. 2018. Optimizing feed composition to meet the nutritional needs of laying hens at minimum cost using Improved Particle Swarm Optimization (IPSO). Journal of Information Technology and Computer Science Development . 2(1): 1-10.
14. Hendrix Genetics. 2022. Isa brown management guide . USA: Hendrix Genetics.
15. Herianto, N., Y. Fenita., U. Santoso., B. Brata and Suharyanto. 2022. Strategy for developing laying hen farms in Lebong Regency. NATURALIS-Journal of Natural Resources and Environmental Management Research . 11(1): 93-101.
16. Hidayat, C and Rahman. 2019. Opportunities for developing phytogenic feed additives as a substitute for antibiotics in broiler chicken rations in Indonesia. Journal of Tropical Animal Husbandry Science and Technology . 6(2): 188-213.
17. Hosseini, R., GEM Lamers., HM Soltani., AH Meijer., HP Spaink and MJM Schaaf. 2016. Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Sciences . 129(18): 3385-3395.
18. Kaur, K and A. Singh. 2014. Drying kinetics and quality characteristics of beetroot slices under hot air followed by microwave finish drying. African Journal of Agricultural Research . 9(10): 36-44.
19. Kumar, S and Brooks, MSL 2017. Use of red beet ( Beta vulgaris L. ) for antimicrobial applications-a critical review. Food and Bioprocess Technology . 11(1): 17-42.
20. Lutfi, MI, H. Nur and Anggraeni. 2015. Effect of adding turmeric ( Curcuma domestica ) extract solution to drinking water on quail ( Coturnix coturnic japonica ) egg production. Indonesian Animal Husbandry Journal . 1(2): 81-88.
21. Mozin, S. 2015. Effect of Using Shallot Waste ( Allium ascolonium L. ) as a Phytobiotic on Broiler Chicken Production Performance. Dissertation . Faculty of Animal Husbandry. Brawijaya University. Poor.
22. Nakayama, M., K. Shimatani., T. Ozawa., N. Shigemune., T. Tsugukuni., D. Tomiyama., M. Kurahachi., A. Nonaka and T. Miyamoto. 2013. A study of the antibacterial mechanism of catechins: Isolation and identification of Escherichia coli cell surface proteins that interact with epigallocatechingallate . Food control . 33(2): 433-439.
23. Nuningtyas, YF 2014. The effect of adding garlic flour ( Allium sativum ) as an additive on the performance of broiler chicken production. Journal of Tropical Livestock . 15(1): 21-30.
24. Putra, T. A., K. A. Safitri., Z. A. N. Bisam and T. A. Shinta. 2022. Identification of secondary metabolite compouns from ethanolic etract of beetroot peel (Beta vulgaris L.). Pharmaceutical Service Scientific Journal. 7(2): 39-44.
25. Ramiah, S. K., Zulkifli., I. Rahim., N. A. A. Ebrahimi and Meng. G. Y. 2014. Effect of two herbal etracts & virginiamycin supplementation on growth performance, intestinal microflora population & fatty acid composition in broiler chickens. Asian-Australasian Journal of Animal Sciences. 27(3): 375-382.
26. Salamatullah, A. M., K. Hayat., M. S. Alkhatam., M. A. Ahmed., S. Arzoo., F. M. Husain., A. M. Al-Dossari., G. Shamlan and L. N. Al-Harbi. 2021. Bioactive and antimicrobial properties of oven-dried beetroot (pulp and peel) using different solvents. Processes. 9: 588.
27. Samour, J. 2015. Diagnostic Value of Hematology in Clinical Avian Medicine . Volume II. Harrison G, Lightfoot TL. Florida: Spix Publishing.
28. Santoso, U. 2022. Efforts to increase consumption of animal protein from livestock in Indonesia. Tropical Farming Newsletter . 3(2): 89-95.
29. Sengul, AY Effect of supplementation of red beetroot powder in quail diets. 2021. Indian Journal of Animal Sciences . 91(1):58-62.
30. Setiawati, T., R. Afnan and N. Ulupi. 2016. Production performance and egg quality of laying hens in litter and cage systems with different cage temperatures. Journal of Animal Production Science and Technology . 4(1): 197-203.
31. Setyorini, D., YC Rahayu and T. Sistyaningrum. 2017. The effect of rinsing red beet root ( Beta vulgaris L ) juice on Streptococcus sp . dental plaque. JDMFS . 2(1): 7-15.
32. Singh, B and BS Hathan. 2014. Chemical composition, functional properties and processing of beetroot-a review. International Journal of Scientific and Engineering Research . 5(6): 79-84.
33. Sjofjan, O. 2003. Study of probiotics ( Aspergillus niger and Bacillus sp ) as feed additives and the implications of their effects on intestinal microflora and production performance of laying hens. Doctoral Dissertation . Postgraduate Faculty, Padjadjaran University, Bandung.
34. Souza, LAZ, HJD Lima., RA Martins., ASA Assuncao., DAN Junior., WF Silva and FG Silva. 2019. Egg yolk color and retinol concentration of eggs from laying hens fed diets containing carrot and beetroot meal. Czech Journal of Animal Science . 64(9): 395-403.
35. Sukrayana, Y., U. Atmomarsono., VD Yunianto and E. Supriyatna. 2016. Improvement of crude protein and crude fiber digestibility of fermented product of palm kernel cake and rice bran mixture for broilers. Journal of Animal Science and Technology . 1(3): 167-172.
36. Suprijatna, E and D. Natawihardja. 2014. Growth of the reproductive organs of laying hens and their impact on egg production performance as a result of providing rations with different levels of protein during the growth period. JITV . 19(3): 260-267.
37. Swiatkiewicz, S., AA Wlosek., J. Krawczyk., W. Szczurek., M. Puchala and D. Jozefak. 2018. Effect of selected feed additives on egg performance and eggshell quality in laying hens fed a diet with standard or decreased calcium content. Animal Science . 18(1): 167-182.
38. Teru, V. 2017. Utilization of Shallot Peel Flour ( Allium ascolonium ) as a Feed Additive on Appearance, Blood Profile and Cholesterol in Laying Quail. Thesis . Brawijaya University Malang. Poor.
39. Waiz, HA 2023. Effects of beetroot juice supplementation on performance, blood-biochemistry of kadaknath chicken raised under farm conditions. Journal of Animal Research . 13(1): 43-48.
40. Weiss, D. J and KJ Wardrop. 2010. Schalm’s Veterinary Hematology . Wiley-Blackwell.
41. Widjastuti, T and R. Kartasudjana. 2006. The effect of restricted feeding and its implications on the performance of Coturnix -coturnix japonica at the first production phase. Journal of the Indonesian Tropical Animal Agriculture . 31(3): 162-166.
42. Widodo, Eko. 2018. Poultry Nutrition Science . Malang: Brawijaya University Press.
43. Wulandari, EC, W. Murningsih and HI Wahyuni. 2012. Calcium and phosphorus deposition in Arabian chicken egg shells by administering various levels of Azolla microphylla. Animal Agriculture Journal . 1(1): 507-520.
44. Xie, Y., W. Yang., F. Tang., X. Chen and L. Ren. 2015. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medical chemistry . 22(1): 132-149.
45. Zhao, X., ZB Yang., WR Yang., Y. Wang., SZ Jiang and GG Zhang. 2011. Effects of ginger root ( Zingiber officinale ) on laying performance and antioidant status of laying hens and on dietary oxidation stability. Poultry Science . 90(8): 1720-1727.