Title : Adulticidal Activities and Synergistic Effects of Citrus Aurantiifolia Peels and Hyptis spicigera Leaves Essential Oils against Anopheles gambiae s.l. (Diptera: Culicidae)

Vol 7 No 3 (2024): Volume 07 Issue 03 March 2024

Article Date Published : 26 March 2024 | Page No.: 1910-1922 | Google Scholar | Crossref doi for this article

Abstract :

Research on new plant-based insecticides meets the need for an alternative to address mosquito resistance to synthetic insecticides. This study assessed the adulticidal activity and synergistic effects of essential oils (EOs) from Citrus aurantiifolia peels and Hyptis spicigera leaves on female adults of Anopheles gambiae s.l. The extraction yields were on the order of 0.17 and 0.11%, respectively, for C. aurantiifolia and H. Spicigera EOs obtained by hydrodistillation. The phytochemical composition of the EOs was analysed by Gas Chromatography coupled with Mass Spectrometry (GC-MS).  GC-MS revealed that the essential oils of C. aurantiifolia and H. spicigera contained high amounts of monoterpene compounds (100% and 77.45%, respectively). Adulticidal activity was assessed using WHO and CDC bottle bioassays at concentrations of 2.5, 5, 7.5 and 10 mg/ml/btl. The EOs of both plants caused significant concentration-dependent Knock down and adulticidal activities. Individually, H. spicigera EOs had more adulticidal effects (LC50 = 4.42 mg/ml/btl) than did C. aurantiifolia EOs (LC50 = 6.79 mg/ml/btl). An EO mixture 25%Ca + 75%Hs from C. aurantiifolia and H. spicigera had synergistic effects (CI = 142.84; SF = 1.42) on female Anopheles gambiae adults. Combinations of the two plant EOs considerably optimize their insecticidal effectiveness.

Keywords :

adulticidal, Anopheles gambiae, essential oils, malaria, synergistic effects.

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Author's Affiliation

   Damas Dainone Ignareki
Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

   Gisele Aurelie Foko Dadji
Department of Biological Sciences, Higher Teacher’s Training College, University of Yaounde I, Yaounde, Cameroon

   Elie Njantou Baudelaire
AGRITECH France, R&D, France & Laboratory of Physics and Chemistry, Jean Baritiot Institute, University of Lorraine, Metz, France & Department of Food Sciences and Nutrition, ENSAI, University of Ngaoundere, Ngaoundere, Cameroon

   Amadou Dicko
Laboratory of Physics and Chemistry, Jean Baritiot Institute, University of Lorraine, Metz, France

   Lame Younoussa
Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

   Dimitri Wendgida Wangrawa
Fundamental and Applied Entomology Laboratory, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso & Norbert Zongo University, Koudougou, Burkina Faso

   Antonio Nkondjio
Malaria Research Laboratory, Organization for Coordinating the Fight against Endemics in Central Africa (OCEAC), Yaoundé, Cameroon.

   Joseph Lebel Tamesse
Department of Biological Sciences, Higher Teacher’s Training College, University of Yaounde I, Yaounde, Cameroon

   Abraham Fomena
Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

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Damas Dainone Ignareki, Gisele Aurelie Foko Dadji, Elie Njantou Baudelaire, Amadou Dicko, Lame Younoussa, Dimitri Wendgida Wangrawa, Antonio Nkondjio, Joseph Lebel Tamesse, Abraham Fomena, 2024

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Adulticidal Activities and Synergistic Effects of Citrus Aurantiifolia Peels and Hyptis spicigera Leaves Essential Oils against Anopheles gambiae s.l. (Diptera: Culicidae). Damas Dainone Ignareki, Gisele Aurelie Foko Dadji, Elie Njantou Baudelaire, Amadou Dicko, Lame Younoussa, Dimitri Wendgida Wangrawa, Antonio Nkondjio, Joseph Lebel Tamesse, Abraham Fomena , 7(3), 1910-1922. Retrieved from https://ijcsrr.org/single-view/?id=15587&pid=15277

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