Abstract :

Dermatophytosis can be caused on by the invasion and infection of keratinized tissues in people and animals via a group of filamentous fungus known as dermatophytes. About a quarter of the world’s population is affected by it which is one of the most prevalent superficial fungal diseases.  Some of these fungi have the capacity to develop complex 3-D biofilm structures, or “biofilm,” which are distinguished by the creation of extracellular polymeric molecules and a heightened drug resistance. The assessment of biofilm now relies on a variety of different methods, which frequently results in various evaluations of the microbial strains’ capacity to create biofilms.

It has only recently been discovered the architecture and growth features of dermatophytic biofilms (Trichophyton spp., Microsporum spp.). Additionally, the structural complexity and lack of research on filamentous fungal biofilms make therapy challenging. Therefore, there is a demand for newer antifungals or methods for treating resistant dermatophytosis to offer an efficient, original, and safe substitute to current treatments. Therefore, this review highlighted on the significance, characterization and evaluation of biofilm that produced from dermatophytes.

 

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Keywords :

Antifungal therapy, biofilm, Dermatophytes, virulence factors.

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References :

1. Pir M., Budak F. and Metiner K. (2022). In vitro antifungal activity of heterocyclic organoboron compounds against Trichophyton mentagrophytes and Microsporum canis obtained from clinical isolates.Brazilian Journal of Microbiology .https://doi.org/10.1007/s42770-022-00777-3
2. Brilhante RSN., Correia EEM., Guedes GM., Pereira VS., Oliveira JS., Bandeira SP., Alencar LP., Andrade ARC., Castelo-Branco DACM., Cordeiro RA., Pinheiro AQ., Chaves LJQ., Neto WAP., Sidrim JJC. and Rocha MFG. 2019. Quantitative and structural analyses of the in vitro and ex vivo biofilm-forming ability of dermatophytes. Journal of Medical Microbiology;66:1045–1052. DOI 10.1099/jmm.0.000528.
3. Gnat S, Łagowski D, Nowakiewicz A. Major challenges and perspectives in the diagnostics and treatment of dermatophyte infections. J Appl Microbiol. 2020; 129: 212–232.
4. Martins, M.P.; Silva, L.G.; Rossi, A.; Sanches, P.R.; Souza, L.D.R.; Martinez-Rossi, N.M. Global Analysis of Cell Wall Genes Revealed Putative Virulence Factors in the Dermatophyte Trichophyton rubrum. Front. Microbiol. 2019, 10, 2168.
5. Martinez-Rossi, N.M.; Bitencourt, T.A.; Peres, N.T.; Lang, E.A.; Gomes, E.V.; Quaresemin, N.R.; Martins, M.P.; Lopes, L.; Rossi, A. Dermatophyte resistance to antifungal drugs: Mechanisms and prospectus. Front. Microbiol. 2018, 9, 1108.
6. Mendes, N.S.; Bitencourt, T.A.; Sanches, P.R.; Silva-Rocha, R.; Martinez-Rossi, N.M.; Rossi, A. Transcriptome-wide survey of gene expression changes and alternative splicing in Trichophyton rubrum in response to undecanoic acid. Sci. Rep. 2018, 8, 2520.
7. Yamada, T.; Yaguchi, T.; Tamura, T.; Pich, C.; Salamin, K.; Feuermann, M.; Monod, M. Itraconazole resistance of Trichophyton rubrum mediated by the ABC transporter TruMDR2. Mycoses 2021, 64, 936–946.
8. Brilhantea RSN., Aguiarb L., Salesb JA., Araujo GS., Pereiraa VS., Pereira-Netoa WA., Pinheirob AQ. , Paix~aoa GC., Cordeiroa RA., Sidrim JJC., Bersanob PRO., Rochaa MFG. and Castelo-Brancoa DSC. (2019). Ex vivo biofilm-forming ability of dermatophytes using dog and cat hair: an ethically viable approach for an infection model. BIOFOULING 2019, VOL. 35, NO. 4, 392–400.
9. Wang, Y.; Lu, C.; Zhao, X.;Wang, D.; Liu, Y.; Sun, S. Antifungal activity and potential mechanism of Asiatic acid alone and in combination with fluconazole against Candida albicans. Biomed. Pharmacother. 2021, 139, 111568.
10. Chen B, Sun Y, Zhang J, Chen R, Zhong X, Wu X, Zheng L and Zhao J (2019) In vitro Evaluation of Photodynamic Effects Against Biofilms of Dermatophytes Involved in Onychomycosis. Front. Microbiol. 10:1228.
11. Alim D., Sircaik S. and Panwar Sl. 2018. The Significance of Lipids to Biofilm Formation in Candida albicans: An Emerging Perspective. J. Fungi 2018, 4, 140.
12. Brilhante RS, de Lima RA, Marques FJ, Silva NF, Caetano ÉP et al. Histoplasma capsulatum in planktonic and biofilm forms: in vitro susceptibility to amphotericin B, itraconazole and farnesol. J Med Microbiol 2015;64:394–399.
13. Gao L, Jiang S, Sun Y, Deng M, Wu Q et al. Evaluation of the effects of photodynamic therapy alone and combined with standard antifungal therapy on planktonic cells and biofilms of Fusarium spp. and Exophiala spp. Front Microbiol 2016;7:617.
14. Burkharta CN, Burkhart CG, Gupta AK. Dermatophytoma: recalcitrance to treatment because of existence of fungal biofilm. J Am Acad Dermatol. 2002; 47(4): 629–31.
15. Chen B, Sun Y, Zhang J, Chen R, Zhong X, Wu X, et al. In vitro evaluation of photodynamic effects against biofilms of dermatophytes involved in onychomycosis. Front Microbiol. 2019; 10: 1228.
16. Sen S, Borah SN, Kandimalla R, Bora A, Deka S. Sophorolipid biosurfactant can control cutaneous dermatophytosis caused by Trichophyton mentagrophytes. Front Microbiol. 2020; 11: 329.
17. Yazdanpanah S., Sasanipoor F., Khodadadi H., Rezaei-Matehkolaei A., Jowkar F., Zomorodian K., Kharazi M., Mohammadi T., Nouripour-Sisakht S., Nasr R.and Motamedi M. (2023). Quantitative analysis of in vitro biofilm formation by clinical isolates of dermatophyte and antibiofilm activity of common antifungal drugs. International Journal of Dermatology.62(1):120-127
18. Azeredo, J.; Azevedo, N.F.; Briandet, R.; Cerca, N.; Coenye, T.; Costa, A.R.; Desvaux, M.; Di Bonaventura, G.; Hébraud, M.; Jaglic, Z.; et al. Critical review on biofilm methods. Crit. Rev. Microbiol. 2017, 43, 313–351.
19. Castelo-Branco D SCM, de Aguiar L, Araujo G S et al. in vitro and ex vivo biofilms of dermatophytes: a new panorama for the study of antifungal drugs. Biofouling. 2020; 36: 783–791.
20. Fanning S, Mitchell AP (2012) Fungal Biofilms. PLoS Pathog 8(4):e1002585.
21. Borghi E, Borgo F, Morace G. Fungal biofilms: update on resistance. Fungal Biofilms and Related Infections. 2016; 3: 37–47.
22. Liu S., Mauff FL., Sheppard DC. and Zhang S. 2022. Filamentous fungal biofilms: Conserved and unique aspects of extracellular matrix composition, mechanisms of drug resistance and regulatory networks in Aspergillus fumigatus. npj Biofilms and Microbiomes (2022) 8:83.
23. Sena S. , Boraha SN., Borac A. and Deka S. (2020). Rhamnolipid exhibits anti-biofilm activity against the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes. Biotechnology Reports 27 (2020) e00516
24. González-Ramírez, A. I., Ramírez-Granillo, A., Medina-Canales, M. G., Rodríguez-Tovar, A. V., & Martínez-Rivera, M. A. (2016). Analysis and description of the stages of Aspergillus fumigatus biofilm formation using scanning electron microscopy. BMC microbiology, 16(1), 1-13.
25. Ramage G., Rajendran R., Sherry L. and Williams C.2012. Fungal Biofilm Resistance. Hindawi Publishing Corporation .International Journal of Microbiology.2012: 14.
26. Costa-Orlandi, C. B., Sardi, J. C., Pitangui, N. S., De Oliveira, H. C., Scorzoni, L., Galeane, M. C., … & Mendes-Giannini, M. J. S. (2017). Fungal biofilms and polymicrobial diseases. J. Fungi, 3(2), 22.
27. Corte L., Pierantoni DC., Tascini C., Roscini L. and Cardinali G.(2019). Biofilm Specific Activity: A Measure to Quantify Microbial Biofilm. Microorganisms 2019, 7, 73.
28. Trafny, E.A.; Lewandowski, R.; Zawistowska-Marciniak, I.; Stepinska, M. Use of MTT assay for determination of the biofilm formation capacity of microorganisms in metalworking fluids. World J. Microbiol. Biotechnol. 2013, 29, 1635–1643.
29. Manavathu, E.K.; Vager, D.L.; Vazquez, J.A. Development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with Aspergillus fumigatus and Pseudomonas aeruginosa. BMC Microbiol. 2014, 14, 53.
30. Trafny, E.A.; Lewandowski, R.; Zawistowska-Marciniak, I.; Stepinska, M. Use of MTT assay for determination of the biofilm formation capacity of microorganisms in metalworking fluids. World J. Microbiol. Biotechnol. 2013, 29, 1635–1643
31. Casagrande Pierantoni, D.; Corte, L.; Roscini, L.; Cardinali, G. High-Throughput Rapid and Inexpensive Assay for Quantitative Determination of Low Cell-Density Yeast Cultures. Microorganisms 2019, 7, 32.
32. Pereira FO. A review of recent research on antifungal agents against dermatophyte biofilms. Medical Mycology, 2021, 59, 313–326.
33. Azeredo J, Azevedo NF, Briandet R et al. Critical review on biofilm methods. Crit Rev Microbiol. 2017; 43: 313–351.
34. Bridier, A.; Meylheuc, T.; Briandet, R. Realistic representation of Bacillus subtilis biofilms architecture using combined microscopy (CLSM, ESEM and FESEM). Micron 2013, 48, 65–69.
35. Karim MT., Ghosh SJ., Chougale RA., Vatkar VS., Deshkar DW. And Narute JV. (2017). Study of Dermatophytes and their Biofilm Production. Journal of Advanced Medical and Dental Sciences Research; 5(11):56-59.