Antiinflammatory Activity of Polyherbal Gel Formulation

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INTRODUCTION
Herbal formulations have reached wide spread acceptability as therapeutic agents like Anti-microbial, Anti-diabetic, Anti-ageing, Anti-arthritic, Anti-depressant, Anti-anxiety and Anti-inflammatory, Anti-HIV, treatment of Cirrhosis, Asthma, Migraine, Alzheimer's disease and Memory enhancing activities.A gel is a solid or semi solid system of at least two constituents, consisting of a condensed mass enclosing and interpenetrated by a liquid.Gels and jellies are composed of small amount of solids dispersed in relatively large amount of liquid, yet they posses more solid-like than liquid-like character.Topical drug delivery systems are gaining increased in popularity, and several drugs have been successfully delivered by this route for both local and systemic action.. 1,2 The presence of a network formed by the interlocking of particles of the gelling agent gives rise to the rigidity of a gel.The nature of the particles and the type of form that is responsible for the linkages determine the structure of the network and the property of the gel. 3he available anti-inflammatory drugs (steroidal and non-steroidal) present a wide range of side effects.Therefore, many studies are being directed to find anti-inflammatory agents from natural sources.Fenugreek (Trigonellafoenum-graecum) and liquorice (Glycyrrhizaglabra) is a plant are used in traditional medicine.Fenugreek act as powerful antioxidant mainly due to the presence of flavonoids and polyphenols. 4,5.7 Even though fenugreek and liquorice has been used for the treatment of inflammation, no report exists on the development of polyherbal gel formulations from hydroalcoholic extract of fenugreek and liquorice.Hence, the present study is aimed at formulating and investigating the effective anti-inflammatory polyherbal gel formulation from the hydroalcoholic extract of Fenugreekand liquorice.

MATERIAL AND METHODS
Collection and Authentification of Plant material: Seeds of Trigonellafoenum-graecum(Fenugreek) and root and stolon of Glycyrrhiza glabra( Liquorice ) were collected from local market and botanical identity was confirmed by Dr.Satish N. Malode.Professor and Head at Govt.Vidarbha Institute of Science and Humanities, Amravati.

DISCUSSION
Topical route for Trigonellafoenum-graecum and Glycyrrhizaglabra was selected up to avoid GIT irritation and to maximize the drug concentration at the site of action.The present study has been under taken with the aim to formulate and evaluate the new polyherbal gel formulation containing 0.3 % hydroalcoholic extract from Trigonellafoenum-graecum and Glycyrrhiza glabra for its topical anti inflammatory activity against carrageenan induced rat paw edema method .All the developed polyherbal gels( F1 to F9 ) were evaluated for their physicochemical properties like appearance, pH values and spreadability, skin irritation test, stability studies and HPTLC methods.In pharmacological evaluation the anti-inflammatory activity was studied using carrageenan induced rat paw edema method.F4 gel showed significant anti-inflammatory activity and results obtained are shown in Table 1 .It showed significant inhibition in carrageenan induced paw edema in Wistar rat models.The results also showed that the antiinflammatory effect of the formulation containing polyherbal gel F4 was better than the other gel formulation.
Anti Inflammatory Study of Gel Formulation Procedure Carrageenan-induced rat paw edema assay in rat The selected samples of Polyherbal gel( F1 to F9) showing promising activity in vivo anti-inflammatory studies using carrageenan induced rat paw edema animal model.The assay performed as edema was induced on the right hind paw by sub plantar injection of 20 ml carrageenan (1 % w/v) in 0.9 % saline.The formulation was topically applied over the inflamed area, (1 % w/v at a dose of 300 mg/kg) after complete induction of inflammation.A control group received vehicle only and a standard group was treated with Indomethacin (20 mg/kg, p.o.) The volume of injected and of the contra lateral paws was measured 1, 3, 5 ,6 h after induction of inflammation, using a plethysmometer on each day till 11 days in M/s.Vineet Analytical Research Laboratories,Pune.The value was expressed as, the percent reduction in volume with respect to the control group of at different time intervals in each day.8,9,10RESULTSResults of carrageenan induced anti-inflammatory activity of Polyherbal Gel formulations containing0.3%hydroalcoholic extract of Fenugreek and Liquorice.

CONCLUSION
In conclusion, the overall result of this study the prepared polyherbal gel formulation F4 with 0.3 % hydroalcoholic extract from Trigonellafoenum-graecum and Glycyrrhizaglabra showed the best formulation with significant anti-inflammatory activity.

Table - 1
. Observation of all formulations ISSN:

2581-8341 Volume 06 Issue 03 March 2023 DOI: 10.47191/ijcsrr/V6-i3-04, Impact Factor: 6.789 IJCSRR @ 2023 www.ijcsrr.org 1885
Pune and the results are shown in following Table -2.The results indicated that the control preparation, test gel, and marketed products did not cause any skin reaction.No reaction, The skin irritation test was carried out in accordance with the Guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals(CPCSEA) * Corresponding Author: A.W.Baitule Volume 06 Issue 03 March 2023 Available at: www.ijcsrr.orgPageNo. 1883-1886 Figure -1 % Inhibition of all formulations with standard drug Skin Irritation StudyThe skin irritation test was conducted for a period of seven days in M/s.Vineet Analytical Research Laboratories,